Acidic Exopolysaccharide Produced from Marine Bacillus amyloliquefaciens 3MS 2017 for the Protection and Treatment of Breast Cancer

Abeer Y Ibrahim; Eman R Youness; Manal G Mahmoud; Mohsen S Asker; Samah A El-Newary

doi:10.1177/1178223420902075

Acidic Exopolysaccharide Produced from Marine Bacillus amyloliquefaciens 3MS 2017 for the Protection and Treatment of Breast Cancer

Breast Cancer (Auckl). 2020 Jan 24:14:1178223420902075. doi: 10.1177/1178223420902075. eCollection 2020.

Authors

Abeer Y Ibrahim¹, Eman R Youness², Manal G Mahmoud³, Mohsen S Asker³, Samah A El-Newary¹

Affiliations

¹ Department of Medicinal and Aromatic Plants Research, National Research Centre, Giza, Egypt.
² Department of Medical Biochemistry, Medical Research Division, National Research Centre, Giza, Egypt.
³ Department of Microbial Biotechnology, National Research Centre, Giza, Egypt.

Abstract

Purpose: This study was planned to investigate the anti-breast-cancer property of acidic exopolysaccharide produced from marine Bacillus amyloliquefaciens 3MS 2017 (BAEPS) in an animal model, which previously showed in-vitro anti-breast-cancer activity, by studying its potential participation in various targeted mechanisms.

Methods: Mammary carcinoma in female Sprague-Dawley rats, both in prophylactic and in curative designs, was chemically induced using 7,12-dimethylebenz-(a)-anthracene (DMBA). B. amyloliquefaciens 3MS 2017 anti-breast-cancer property was evaluated by studying its effects on cancer-growth-rate-limiting enzymes (aromatase and Na⁺/K⁺ ATPase), sexual hormones (estrogen and progesterone), antioxidant and inflammatory biomarkers (cyclooxygenase-1; COX-1 and cyclooxygenase-2; COX-2). The incidence of breast cancer by DMBA was dependent on the level of carcinoembryonic antigen (CEA) and aromatase.

Results: 7,12-Dimethylebenz-(a)-anthracene female rats were characterized by a significant increase in cancer-related biomarkers with an increase of oxidative stress biomarkers, in comparison with the negative control. Potent BAEPS anticancer activity on DMBA rats was exhibited either as a prophylactic or as a curative agent, which appeared via restoring the aromatase and Na⁺/K⁺ ATPase subunits levels and CEA close to the normal level. Besides, BAEPS modulated a sexual hormone, in comparison with the cancer control group (P ⩽ .05). B. amyloliquefaciens 3MS 2017 selectively inhibited COX-2 in parallel with promising antioxidant properties. The curative characters of BAEPS were more promising than the prophylactic.

Conclusion: The anti-breast-cancer characters accompanied with a good safety margin may be attributed to its inhibitory effect on cancer-growth-rate-limiting enzymes, estrogen production, COX-2 level and lipid peroxidation, concurrent with enhancing COX-1 level, progesterone production, and antioxidant status.

Keywords: Acidic exopolysaccharide; Bacillus amyloliquefaciens 3MS 2017; anti-inflammatory; antioxidant; breast cancer; cancer-growth-rate-limiting enzymes.