LncRNA Gm12664-001 ameliorates nonalcoholic fatty liver through modulating miR-295-5p and CAV1 expression

Qiao Zhang; Jiemei Wang; Hongyin Li; Yuan Zhang; Xia Chu; Jianjun Yang; Ying Li

doi:10.1186/s12986-020-0430-z

LncRNA Gm12664-001 ameliorates nonalcoholic fatty liver through modulating miR-295-5p and CAV1 expression

Nutr Metab (Lond). 2020 Feb 4:17:13. doi: 10.1186/s12986-020-0430-z. eCollection 2020.

Authors

Qiao Zhang^{1

2}, Jiemei Wang¹, Hongyin Li¹, Yuan Zhang¹, Xia Chu¹, Jianjun Yang³, Ying Li¹

Affiliations

¹ 1Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, 150086 China.
² 2Department of Public Health College, Kunming Medical University, Kunming, 650550 China.
³ 3School of Public Health, Ningxia Medical University, Yinchuan, 750004 China.

Abstract

Background: Our study aims to investigate the mechanisms of lncRNA Gm12664-001 improved hepatic lipid accumulation-initiated NAFLD via regulating miR-295-5p and CAV1 in AML12 cells.

Methods: The animals were divided into normal control (NC) group and high fat diet (HFD) group (20 mice per group) for 8w. The steatotic liver was measured by hematoxylin eosin (HE) staining and kits. We performed systematical analyses on hepatic expression profiles of long noncoding RNAs (lncRNAs) and microRNAs in a high-fat diet (HFD)-induced steatotic animal model. The expression profile of targets was confirmed by bioinformatics analysis, luciferase assay, RT-PCR and western blot in AML12 cells.

Results: HFD treatment markedly observed hepatic fatty degeneration with primarily fat vacuoles, and increased TG level compared with control. According to microarray data, we found that transfection of Gm12664-001 siRNA (siRNA-118,306) obviously enhanced TG accumulation and repressed CAV1 in AML12 cells. Furthermore, the TG accumulation markedly increased by siRNA-mediated knockdown of CAV1 in AML12 cells. By bioinformatics prediction, AML12 cells were transfected of siRNA-118,306 obviously upregulated miR-295-5p. Transfection of miR-295-5p mimics significantly increased TG accumulation and obviously suppressed the target CAV1.

Conclusions: The results revealed that lncRNA Gm12664-001 attenuated hepatic lipid accumulation through negatively regulating miR-295-5p and enhancing CAV1 expression in AML12 cells.

Keywords: CAV1; Gm12664–001; Nonalcoholic fatty liver; miR-295-5p.