Abstract
[Tm(DPA)3]3- was used to generate multiple, paramagnetic nuclear Overhauser effect NMR spectra of cationic peptides when weakly bound to a lipopolysaccharide micelle. Increased spectral resolution combined with a marked increase in the number of distance restraints yielded high resolution structures of polymyxin and MSI-594 in the liposaccharide bound state.
MeSH terms
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Antimicrobial Cationic Peptides / chemistry*
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Indicators and Reagents / chemistry
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Lanthanoid Series Elements / chemistry*
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Micelles*
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Nuclear Magnetic Resonance, Biomolecular / methods*
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Peptides / chemistry
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Polymyxin B / chemistry
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Protein Conformation
Substances
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Antimicrobial Cationic Peptides
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Indicators and Reagents
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Lanthanoid Series Elements
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MSI 594
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Micelles
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Peptides
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Polymyxin B