Identification of Binding Epitopes of a Fluorinated Compound Bound to Proteins Using 1H and 19F NMR Spectroscopy

Anal Sci. 2020 Jul 10;36(7):881-883. doi: 10.2116/analsci.19N033. Epub 2020 Feb 7.

Abstract

1H/19F NMR-based screening methods were applied to a human serum albumin-fleroxacin complex. Fleroxacin contains three fluorine atoms in a molecule, which is suitable as a model fluorinated compound for NMR analysis with 1H and 19F detection. The 19F{1H} and 1H{1H} saturation transfer difference spectra were acquired and the 1H/19F spin-lattice relaxation rates were measured with and without any selective irradiation of protein resonance to identify the binding epitopes of fleroxacin. Because several 1H signals of fleroxacin resonated close to water, its precise signal intensities were unavailable. The 19F NMR-based screening methods successfully provide complementary information, indicating its importance in the analysis of fluorinated compounds.

Keywords: 19F NMR; NMR-based screening; fluorinated compounds.

MeSH terms

  • Binding Sites
  • Epitopes / chemistry*
  • Fleroxacin / analysis*
  • Fluorine
  • Humans
  • Hydrogen
  • Magnetic Resonance Spectroscopy
  • Nuclear Magnetic Resonance, Biomolecular*
  • Serum Albumin, Human / chemistry*

Substances

  • Epitopes
  • Fluorine
  • Hydrogen
  • Fleroxacin
  • Serum Albumin, Human