Polydopamine nanospheres as high-affinity signal tag towards lateral flow immunoassay for sensitive furazolidone detection

Sijie Liu; Leina Dou; Xiaolin Yao; Wentao Zhang; Bingxin Zhao; Zonghan Wang; Yanwei Ji; Jing Sun; Baocheng Xu; Daohong Zhang; Jianlong Wang

doi:10.1016/j.foodchem.2020.126310

Polydopamine nanospheres as high-affinity signal tag towards lateral flow immunoassay for sensitive furazolidone detection

Food Chem. 2020 Jun 15:315:126310. doi: 10.1016/j.foodchem.2020.126310. Epub 2020 Jan 30.

Authors

Sijie Liu¹, Leina Dou¹, Xiaolin Yao¹, Wentao Zhang¹, Bingxin Zhao¹, Zonghan Wang¹, Yanwei Ji¹, Jing Sun², Baocheng Xu³, Daohong Zhang⁴, Jianlong Wang⁵

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
² Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.
³ College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China.
⁴ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: zhangdh@nwsuaf.edu.cn.
⁵ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: wanglong79@nwsuaf.edu.cn.

PMID: 32036292
DOI: 10.1016/j.foodchem.2020.126310

Abstract

Currently, the low sensitivity and poor binding stability of detection probe prepared via electrostatic adsorption have become the dilemmas of colloidal gold-based lateral flow immunoassays (Au-LFIAs). In this connection, polydopamine nanospheres (PDA NPs) with an eminent covalent connectivity property were introduced as a promising substitute to improve the stability of probe and sensitivity of LFIA. Whereafter, the PDA NPs-based LFIA was applied for the monitoring of furazolidone (FZD) in food samples because of the potential carcinogenic/mutagenic effects to human of its metabolite (3-amino-2-oxazolidinone, AOZ). Compared with electrostatic adsorption, the binding stability of PDA NPs-based probes was superior. And, as expected, the PDA NPs-based LFIA biosensor exhibited higher sensitivity than that of the Au-LFIA with a detection limit of 3.5 ng mL^-1 for AOZ by naked-eye readout. Based on the significant enhanced binding stability and sensitivity, the PDA NPs-based LFIA is of certain spreading value for detecting other analytes.

Keywords: Binding stability; Furazolidone; Lateral flow immunoassay; Polydopamine nanospheres.

MeSH terms

Biosensing Techniques
Furazolidone / analysis*
Humans
Immunoassay*
Indoles / chemistry*
Limit of Detection
Nanospheres / chemistry*
Polymers / chemistry*

Substances

Indoles
Polymers
polydopamine
Furazolidone