Microbiologically influenced corrosion of 304 stainless steel by nitrate reducing Bacillus cereus in simulated Beijing soil solution

Si Yu; Yuntian Lou; Dawei Zhang; Enze Zhou; Zhong Li; Cuiwei Du; Hongchang Qian; Dake Xu; Tingyue Gu

doi:10.1016/j.bioelechem.2020.107477

Microbiologically influenced corrosion of 304 stainless steel by nitrate reducing Bacillus cereus in simulated Beijing soil solution

Bioelectrochemistry. 2020 Jun:133:107477. doi: 10.1016/j.bioelechem.2020.107477. Epub 2020 Jan 31.

Authors

Si Yu¹, Yuntian Lou¹, Dawei Zhang¹, Enze Zhou², Zhong Li³, Cuiwei Du⁴, Hongchang Qian¹, Dake Xu⁵, Tingyue Gu³

Affiliations

¹ Corrosion and Protection Center, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China.
² Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China.
³ Department of Chemical and Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, OH 45701, USA.
⁴ Corrosion and Protection Center, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China; Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China. Electronic address: dcw@ustb.edu.cn.
⁵ Corrosion and Protection Center, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China; Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China. Electronic address: xudake@mail.neu.edu.cn.

PMID: 32035394
DOI: 10.1016/j.bioelechem.2020.107477

Abstract

In this work, microbiologically influenced corrosion (MIC) of 304 stainless steel (SS) caused by Bacillus cereus was investigated by electrochemical measurements and surface analyses in simulated Beijing soil solution under aerobic condition. The nitrate-reducing bacterium (NRB), B. cereus, was isolated from Beijing soil and identified using 16S rDNA. Confocal laser scanning microscopy (CLSM) images showed that the largest pit depths on 304 SS with and without B. cereus after 14 days of incubation were 7.17 and 4.59 μm, respectively, indicating that pitting corrosion was accelerated by B. cereus. X-ray photoelectron spectroscopy (XPS) and energy dispersive spectrometry (EDS) results revealed that B. cereus and its metabolic products were detrimental to the integrity of the passive film on 304 SS. The electrochemical results showed that B. cereus significantly reduced the corrosion resistance of 304 SS and accelerated the anodic dissolution reaction, thereby speeding up the corrosion process.

Keywords: Microbiologically influenced corrosion; Nitrate-reducing bacterium; Soil corrosion; Stainless steel.

MeSH terms

Bacillus cereus / physiology*
Beijing
Biofilms
Corrosion
Electrochemical Techniques
Electrodes
Nitrates / metabolism*
Oxidation-Reduction
Soil / chemistry
Soil Microbiology*
Stainless Steel / chemistry*

Substances

Nitrates
Soil
Stainless Steel