Comparative study of DAM, Dof, and WRKY gene families in fourteen species and their expression in Vitis vinifera

Lingfei Shangguan; Mengxia Chen; Xiang Fang; Zhenqiang Xie; Kekun Zhang; Ting Zheng; Yunfeng Pu; Jinggui Fang

doi:10.1007/s13205-019-2039-3

Comparative study of DAM, Dof, and WRKY gene families in fourteen species and their expression in Vitis vinifera

3 Biotech. 2020 Feb;10(2):72. doi: 10.1007/s13205-019-2039-3. Epub 2020 Jan 27.

Authors

Lingfei Shangguan^#^{1

2}, Mengxia Chen^#^{1

2}, Xiang Fang¹, Zhenqiang Xie^{1

2

3}, Kekun Zhang^{1

2}, Ting Zheng^{1

2}, Yunfeng Pu⁴, Jinggui Fang^{1

2}

Affiliations

¹ 1Department of Horticulture, Nanjing Agricultural University, Nanjing, 210095 Jiangsu People's Republic of China.
² Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, 210095 People's Republic of China.
³ Department of Agriculture and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212499 Jiangsu People's Republic of China.
⁴ 4Department of Life Science, Tarim University, Xinjiang Uygur Autonomous region, Aral city, 843300 People's Republic of China.

^# Contributed equally.

Abstract

Bud dormancy is one of the most important defensive mechanisms through which plants resist cold stress during harsh winter weather. DAM, Dof, and WRKY have been reported to be involved in many biological processes, including bud dormancy. In the present study, grapevine (Vitis vinifera) and other thirteen plants (six woody plants and seven herbaceous plants) were analyzed for the quantity, sequence structure, and evolution patterns of their DAM, Dof, and WRKY gene family members. Moreover, the expression of VvDAM, VvDof, and VvWRKY genes was also investigated. Thus, 51 DAM, 1,205 WRKY, and 489 Dof genes were isolated from selected genomes, while 5 DAM, 114 WRKY, and 50 Dof duplicate gene pairs were identified in 10 genomes. Moreover, WGD and segmental duplication events were associated with the majority of the expansions of Dof and WRKY gene families. The VvDAM, VvDof, and VvWRKY genes significantly differentially expressed throughout bud dormancy outnumbered those significantly differentially expressed throughout fruit development or under abiotic stresses. Interestingly, multiple stress responsive genes were identified, such as VvDAM (VIT_00s0313g00070), two VvDof genes (VIT_18s0001g11310 and VIT_02s0025g02250), and two VvWRKY genes (VIT_07s0031g01710 and VIT_11s0052g00450). These data provide candidate genes for molecular biology research investigating bud dormancy and responses to abiotic stresses (namely salt, drought, copper, and waterlogging).

Keywords: 14 species; Bud dormancy; DAM; Dof; Vitis vinifera; WRKY.