Abstract
Deformation of vacuolar membranes mediated by endosomal sorting complex required for transport (ESCRT) is necessary for microautophagy. Target of rapamycin complex 1 (TORC1) protein kinase negatively regulates ESCRT-0 (Vps27-Hse1) recruitment onto vacuolar membranes and microautophagy induction. However, whether and how protein phosphatase regulates these events is unknown. Here, we show that the TORC1-downstream protein phosphatase PP2A-Cdc55 is important for these events after TORC1 inactivation in budding yeast. Loss of PP2A-Cdc55 compromised vacuolar localization of Hse1, but not Vps27. This study revealed that the orchestrated action of PP2A induces microautophagy upon TORC1 inactivation.
Keywords:
ESCRT-0; Hse1; Microautophagy; PP2A; TORC1; Vps27.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Cycle Proteins / metabolism
-
Endosomal Sorting Complexes Required for Transport / metabolism*
-
Intracellular Membranes / metabolism*
-
Mechanistic Target of Rapamycin Complex 1 / metabolism*
-
Microautophagy*
-
Protein Binding
-
Protein Phosphatase 2 / metabolism
-
Receptors, Cytoplasmic and Nuclear / metabolism
-
Saccharomyces cerevisiae / cytology*
-
Saccharomyces cerevisiae / metabolism*
-
Saccharomyces cerevisiae Proteins / metabolism*
-
Vacuoles / metabolism*
Substances
-
CDC55 protein, S cerevisiae
-
Cell Cycle Proteins
-
Endosomal Sorting Complexes Required for Transport
-
Hse1 protein, S cerevisiae
-
Receptors, Cytoplasmic and Nuclear
-
Saccharomyces cerevisiae Proteins
-
VPS27 protein, S cerevisiae
-
Mechanistic Target of Rapamycin Complex 1
-
Protein Phosphatase 2