Fabrication and application of zwitterionic phosphorylcholine functionalized monoliths with different hydrophilic crosslinkers in hydrophilic interaction chromatography

Qiqin Wang; Qiaoxuan Zhang; Hao Huang; Pengwei Zhao; Lingjue Sun; Kun Peng; Xiao Liu; Meng Ruan; Huikai Shao; Jacques Crommen; Pei Yu; Zhengjin Jiang

doi:10.1016/j.aca.2019.12.016

Fabrication and application of zwitterionic phosphorylcholine functionalized monoliths with different hydrophilic crosslinkers in hydrophilic interaction chromatography

Anal Chim Acta. 2020 Mar 8:1101:222-229. doi: 10.1016/j.aca.2019.12.016. Epub 2019 Dec 9.

Authors

Qiqin Wang¹, Qiaoxuan Zhang², Hao Huang³, Pengwei Zhao⁴, Lingjue Sun³, Kun Peng³, Xiao Liu³, Meng Ruan³, Huikai Shao³, Jacques Crommen⁵, Pei Yu⁶, Zhengjin Jiang⁷

Affiliations

¹ Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization, Innovative Drug Development of Chinese Ministry of Education (MOE), School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou, 510632, China; Department of Pharmacy, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, 510632, China.
² Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
³ Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China.
⁴ Traditional Chinese Medicine College, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
⁵ Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China; Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmaceutical Sciences, CIRM, University of Liege, CHU B36, B-4000, Liege, Belgium.
⁶ Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization, Innovative Drug Development of Chinese Ministry of Education (MOE), School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou, 510632, China; Department of Pharmacy, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, 510632, China. Electronic address: pennypeiyu@163.com.
⁷ Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization, Innovative Drug Development of Chinese Ministry of Education (MOE), School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou, 510632, China; Department of Pharmacy, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, 510632, China. Electronic address: jzjjackson@hotmail.com.

PMID: 32029114
DOI: 10.1016/j.aca.2019.12.016

Abstract

In this study, a series of zwitterionic phosphorylcholine functionalized monolithic columns were fabricated via the thermally initiated co-polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and different hydrophilic crosslinkers, including 1,4-bis(acryloyl)piperazine (PDA), N,N'-methylenebisacrylamide (MBA) and 2-(methacryloyloxy)ethyl-[N-(2-methacryloyloxy)ethyl]phosphorylcholine (MMPC). The physicochemical and chromatographic properties of these MPC functionalized monoliths, including column morphology, pore size distribution, permeability, column efficiency, retention mechanism and ζ-potential analysis, were systematically compared. Furthermore, the influence of the crosslinker on the chromatographic performance of these MPC functionalized monoliths was evaluated. The chromatographic results indicate that the polarity of MPC functionalized monoliths may be related to the polarity of the crosslinker, which further affects the column selectivity and efficiency. A particularly high column efficiency (88,000 plates/m) was obtained on the novel poly(MPC-co-MMPC) monolith at optimum linear velocity using thiourea as test analyte. Compared to the poly(MPC-co-MBA) and poly(MPC-co-PDA) monoliths, the poly(MPC-co-MMPC) monolith exhibited higher separation selectivity for polar analytes, including nucleobases, nucleosides and benzoic acid derivatives. Moreover, 24 N-glycopeptides could be detected after enrichment with the poly(MPC-co-MMPC) versus 19 and 10 N-glycopeptides with the poly(MPC-co-MBA) and poly(MPC-co-PDA) monoliths, and no N-glycopeptide without enrichment. Therefore, MMPC has a great potential as a new and alternative hydrophilic crosslinker for the development of zwitterionic polymeric monoliths.

Keywords: Hydrophilic crosslinker; Hydrophilic interaction chromatography; Phosphorylcholine; Separation science; Zwitterionic polymeric monolith.