Genetic association analysis identifies a role for ANO5 in prostate cancer progression

Chia-Cheng Yu; Lih-Chyang Chen; Chao-Yuan Huang; Victor C Lin; Te-Ling Lu; Cheng-Hsueh Lee; Shu-Pin Huang; Bo-Ying Bao

doi:10.1002/cam4.2909

Genetic association analysis identifies a role for ANO5 in prostate cancer progression

Cancer Med. 2020 Apr;9(7):2372-2378. doi: 10.1002/cam4.2909. Epub 2020 Feb 6.

Authors

Chia-Cheng Yu^{1

2

3}, Lih-Chyang Chen⁴, Chao-Yuan Huang⁵, Victor C Lin^{6

7}, Te-Ling Lu⁸, Cheng-Hsueh Lee⁹, Shu-Pin Huang^{9

10

11

12}, Bo-Ying Bao^{8

13

14}

Affiliations

¹ Division of Urology/Transplant Surgery, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
² Department of Urology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
³ Department of Pharmacy, College of Pharmacy and Health Care, Tajen University, Pingtung, Taiwan.
⁴ Department of Medicine, Mackay Medical College, New Taipei City, Taiwan.
⁵ Department of Urology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
⁶ Department of Urology, E-Da Hospital, Kaohsiung, Taiwan.
⁷ School of Medicine for International Students, I-Shou University, Kaohsiung, Taiwan.
⁸ Department of Pharmacy, China Medical University, Taichung, Taiwan.
⁹ Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
¹⁰ Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
¹¹ Department of Urology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
¹² Center for Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan.
¹³ Sex Hormone Research Center, China Medical University Hospital, Taichung, Taiwan.
¹⁴ Department of Nursing, Asia University, Taichung, Taiwan.

Abstract

Anoctamins were originally identified as a family of calcium-activated chloride channels, but recently their roles in the development of different types of malignancies were suggested. Here, we evaluated the associations between 211 common single-nucleotide polymorphisms in 10 anoctamin genes with biochemical recurrence (BCR) after radical prostatectomy (RP) for localized prostate cancer. Four SNPs (ANO4 rs585335, ANO5 rs4622263, ANO7 rs62187431, and ANO10 rs118005571) remained significantly associated with BCR after multiple test correction (P < .05 and q = 0.232) and adjustment for known prognostic factors. Expression quantitative trait loci analysis found that ANO5 rs4622263 C and ANO10 rs118005571 C alleles were associated with decreased mRNA expression levels. Moreover, lower expression of ANO5 was correlated with more advanced tumors and poorer outcomes in two independent prostate cancer cohorts. Taken together, ANO5 rs4622263 was associated with BCR, and ANO5 gene expression was correlated with patient prognosis, suggesting a pivotal role for ANO5 in prostate cancer progression.

Keywords: anoctamin; biomarker; prognosis; progression; prostate cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Anoctamins / genetics*
Biomarkers, Tumor / genetics*
Disease Progression
Follow-Up Studies
Genetic Testing
Humans
Male
Middle Aged
Patient Selection
Polymorphism, Single Nucleotide*
Prognosis
Prostatectomy / mortality*
Prostatic Neoplasms / genetics
Prostatic Neoplasms / pathology*
Prostatic Neoplasms / surgery
Quantitative Trait Loci*
Survival Rate

Substances

ANO5 protein, human
Anoctamins
Biomarkers, Tumor