LncRNA WT1-AS Downregulates Survivin by Upregulating miR-203 in Papillary Thyroid Carcinoma

Fei Le; Ping Luo; Qian Ouyang; Xiaoming Zhong

doi:10.2147/CMAR.S232294

LncRNA WT1-AS Downregulates Survivin by Upregulating miR-203 in Papillary Thyroid Carcinoma

Cancer Manag Res. 2020 Jan 20:12:443-449. doi: 10.2147/CMAR.S232294. eCollection 2020.

Authors

Fei Le¹, Ping Luo², Qian Ouyang³, Xiaoming Zhong⁴

Affiliations

¹ Department of Head and Neck Surgery, Jiangxi Province Cancer Hospital, Nanchang City, Jiangxi Province, 330029, People's Republic of China.
² Department of Surgical Oncology, Nanchang Third Hospital Surgical Oncology, Nanchang City, Jiangxi Province, 330002, People's Republic of China.
³ Department of Intensive Medicine, Jiangxi Province Cancer Hospital, Nanchang City, Jiangxi Province, 330029, People's Republic of China.
⁴ Department of Tumor Radiotherapy, Jiangxi Province Cancer Hospital, Nanchang City, Jiangxi Province, 330029, People's Republic of China.

Abstract

Objective: This study aimed to assessment the functions of lncRNA WT1-AS in papillary thyroid carcinoma (PTC).

Methods: Expression levels of WT1-AS in PTC and non-tumor tissues from 66 PTC patients were measured and compared by performing qPCR and paired t test, respectively. Cell proliferation (CCK-8) assay was performed to evaluate the effects of the overexpression of WT1-AS, miR-203 and survivin on the proliferation of IHH-4 (a human PTC cell line) cells.

Results: We found that WT1-AS was significantly downregulated in PTC and associated with clinical stages. In PTC tissues, WT1-AS was negatively correlated with survivin but positively correlated with miR-203. In PTC cells, WT1-AS overexpression led to significantly upregulated miR-203 and downregulated survivin. MiR-203 overexpression failed to affect WT1-AS but downregulated survivin. Cell proliferation assay showed that overexpression of WT1-AS and miR-203 led to decreased, while survivin overexpression led to increased proliferation of PTC cells. In addition, survivin overexpression attenuated the effects of WT1-AS and miR-203 overexpression.

Conclusion: Therefore, WT1-AS may downregulate survivin by upregulating miR-203 in PTC to inhibit cancer cell proliferation.

Keywords: WT1-AS; miR-203; papillary thyroid carcinoma; survivin.