YAP-depleted iPSC MUSIi012-A-2 maintained all normal stem cell characteristics

Chanchao Lorthongpanich; Chuti Laowtammathron; Nittaya Jiamvoraphong; Pimonwan Srisook; Pimjai Chingsuwanrote; Phatchanat Klaihmon; Nattaya Damkham; Papussorn Terbto; Supaporn Waeteekul; Yaowalak U-Pratya; Surapol Issaragrisil

doi:10.1016/j.scr.2020.101723

YAP-depleted iPSC MUSIi012-A-2 maintained all normal stem cell characteristics

Stem Cell Res. 2020 Mar:43:101723. doi: 10.1016/j.scr.2020.101723. Epub 2020 Jan 27.

Affiliations

¹ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Electronic address: chanchao.lor@mahidol.ac.th.
² Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
³ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁴ Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁵ Division of Medical Genetics, Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁶ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁷ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Bangkok Hematology Center, Wattanosoth Hospital, BDMS Center of Excellence for Cancer, Bangkok, Thailand.

PMID: 32018207
DOI: 10.1016/j.scr.2020.101723

Abstract

Yes-associated protein (YAP) is an important transcriptional coactivator in the Hippo signaling pathway. Using CRISPR/Cas9 technology, we established a stable YAP-knockdown (YAP-KD) induced pluripotent stem cell (iPSC) from the MUSIi012-A cell line. The YAP-KD iPSC MUSIi012-A-2 maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and it maintained the normal karyotype.

Keywords: Hippo signaling pathway; YAP; iPSC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Cycle Proteins / genetics*
Cell Differentiation
Female
Gene Editing / methods*
Humans
Induced Pluripotent Stem Cells / metabolism*
Transcription Factors / genetics*

Substances

Cell Cycle Proteins
Transcription Factors
YY1AP1 protein, human