Osteoarticular Infection in Three Young Thoroughbred Horses Caused by a Novel Gram Negative Cocco-Bacillus

Bernard J Hudson; Catherine Chicken; Anna Blishen; Kristen H Todhunter; Angela P Begg; Leonie Chan; Thomas Karagiannis; Benjamin Raymond; Daniel Bogema; Angus R Adkins; Christopher B O'Sullivan; Brendon A O'Rourke; Piklu Roy Chowdhury; Steven P Djordjevic; Ian G Charles; Andrew Edgar; Katerina Mitsakos

doi:10.1155/2020/9785861

Osteoarticular Infection in Three Young Thoroughbred Horses Caused by a Novel Gram Negative Cocco-Bacillus

Case Rep Vet Med. 2020 Jan 19:2020:9785861. doi: 10.1155/2020/9785861. eCollection 2020.

Authors

Bernard J Hudson¹, Catherine Chicken², Anna Blishen², Kristen H Todhunter^{3

4}, Angela P Begg³, Leonie Chan¹, Thomas Karagiannis¹, Benjamin Raymond⁵, Daniel Bogema⁶, Angus R Adkins², Christopher B O'Sullivan⁷, Brendon A O'Rourke⁶, Piklu Roy Chowdhury⁵, Steven P Djordjevic⁵, Ian G Charles^{5

8}, Andrew Edgar⁹, Katerina Mitsakos^{1

5}

Affiliations

¹ NSW Health Pathology, Department of Microbiology & Infectious Diseases, Royal North Shore Hospital, St Leonards, Sydney, New South Wales 2065, Australia.
² Scone Equine Hospital, 106 Liverpool Street, Scone, New South Wales 2337, Australia.
³ Vetnostics Laverty Pathology, 60 Waterloo Rd, Macquarie Park, Sydney, New South Wales 2113, Australia.
⁴ Newcastle Equine Centre & Newcastle Equine Rehabilitation & Reproduction Centre, The Old Tote Building, Broadmeadow Racecourse, New South Wales 2292, Australia.
⁵ The ithree Institute, University of Technology, 15 Broadway, Ultimo, Sydney, New South Wales 2007, Australia.
⁶ Department of Primary Industry, Elizabeth Macarthur Agricultural Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia.
⁷ Randwick Equine Centre, 3 Jane St, Randwick, Sydney, New South Wales 2031, Australia.
⁸ Quadrum Institute Bioscience-Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, UK.
⁹ Newmarket Equine Hospital, Cambridge Road, Newmarket, Suffolk CB8 OFG, UK.

Abstract

We describe three cases of osteoarticular infection (OAI) in young thoroughbred horses in which the causative organism was identified by MALDI-TOF as Kingella species. The pattern of OAI resembled that reported with Kingella infection in humans. Analysis by 16S rRNA PCR enabled construction of a phylogenetic tree that placed the isolates closer to Simonsiella and Alysiella species, rather than Kingella species. Average nucleotide identity (ANI) comparison between the new isolate and Kingella kingae and Alysiella crassa however revealed low probability that the new isolate belonged to either of these species. This preliminary analysis suggests the organism isolated is a previously unrecognised species.

Publication types

Case Reports