Abstract
This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.
© 2020 Cold Spring Harbor Laboratory Press.
MeSH terms
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Anions
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Centrifugation / methods*
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Chromatography, Ion Exchange / instrumentation
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Chromatography, Ion Exchange / methods*
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Dependovirus / genetics
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Dependovirus / isolation & purification*
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Genetic Vectors / genetics
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Genetic Vectors / isolation & purification*
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Hydrogen-Ion Concentration
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Recombination, Genetic
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Reproducibility of Results
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Temperature
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Triiodobenzoic Acids / chemistry*
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Viral Genome Packaging
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Virion / genetics*
Substances
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Anions
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Triiodobenzoic Acids
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iodixanol