Enrichment of Fully Packaged Virions in Column-Purified Recombinant Adeno-Associated Virus (rAAV) Preparations by Iodixanol Gradient Centrifugation Followed by Anion-Exchange Column Chromatography

Cold Spring Harb Protoc. 2020 Feb 3;2020(2):095638. doi: 10.1101/pdb.prot095638.

Abstract

This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

MeSH terms

  • Anions
  • Centrifugation / methods*
  • Chromatography, Ion Exchange / instrumentation
  • Chromatography, Ion Exchange / methods*
  • Dependovirus / genetics
  • Dependovirus / isolation & purification*
  • Genetic Vectors / genetics
  • Genetic Vectors / isolation & purification*
  • Hydrogen-Ion Concentration
  • Recombination, Genetic
  • Reproducibility of Results
  • Temperature
  • Triiodobenzoic Acids / chemistry*
  • Viral Genome Packaging
  • Virion / genetics*

Substances

  • Anions
  • Triiodobenzoic Acids
  • iodixanol