Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)

Hyejin An; Ick-Hyun Jo; Youn-Lee Oh; Kab-Yeul Jang; Won-Sik Kong; Jwa-Kyung Sung; Yoon-Sup So; Jong-Wook Chung

doi:10.1080/12298093.2019.1667131

Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)

Mycobiology. 2019 Sep 27;47(4):527-532. doi: 10.1080/12298093.2019.1667131. eCollection 2019.

Authors

Hyejin An¹, Ick-Hyun Jo², Youn-Lee Oh², Kab-Yeul Jang², Won-Sik Kong², Jwa-Kyung Sung³, Yoon-Sup So³, Jong-Wook Chung¹

Affiliations

¹ Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Republic of Korea.
² Department of Herbal Crop Research, Rural Development Administration, National Institute of Horticultural and Herbal Science, Eumseong, Republic of Korea.
³ Department of Crop Science, Chungbuk National University, Cheongju, Republic of Korea.

Abstract

We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (M_AF) and the average number of alleles (N_A) were 0.50 and 5.47, respectively. The average number of genotypes (N_G), observed heterozygosity (H_O), expected heterozygosity (H_E), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that M_AF was negatively correlated with N_G (-0.683), N_A (-0.600), H_O (-0.584), and PIC (-0.941). N_G, N_A, H_O, and PIC were positively correlated with other polymorphic parameters except for M_AF. UPGMA clustering showed that 26 A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.

Keywords: Button mushroom; cluster analysis; genetic diversity; simple sequence repeat marker.

Grants and funding

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Golden Seed Project, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) [213007-05-3-SBJ30].