Fetal bovine serum attenuating perfluorooctanoic acid-inducing toxicity to multiple human cell lines via albumin binding

Abstract

Perfluorooctanoic acid (PFOA), as a typical emerging organic pollutant, can interact with serum albumin. However, it remains to characterize the binding of PFOA with serum albumin and to address the role of this interaction in related toxic effects. We aimed to characterize the interaction between PFOA and albumin for understanding the effects of this interaction on the uptake, distribution, and toxicity of PFOA in human cells. The results showed that viable cell count was significantly enhanced by addition of fetabl bovine serum (FBS) into cell culture medium with 300 μM PFOA treatment. PFOA mainly existed as complexed with FBS, at FBS concentration > 10%, which substantially reduced the absorption efficiency of all cell lines to PFOA. The majority of PFOA was accumulated in the cytosolic fraction, followed by nuclei, and mitochondria. Conclusively, our study suggests that the complexation of organic contaminants with proteins might mitigate their toxicity by reducing cellular uptake.

Keywords: Distribution and serum albumin; PFOA; Toxicity.