Cultivating Chlorella sorokiniana AK-1 with swine wastewater for simultaneous wastewater treatment and algal biomass production

Chun-Yen Chen; En-Wei Kuo; Dillirani Nagarajan; Shih-Hsin Ho; Cheng-Di Dong; Duu-Jong Lee; Jo-Shu Chang

doi:10.1016/j.biortech.2020.122814

Cultivating Chlorella sorokiniana AK-1 with swine wastewater for simultaneous wastewater treatment and algal biomass production

Bioresour Technol. 2020 Apr:302:122814. doi: 10.1016/j.biortech.2020.122814. Epub 2020 Jan 20.

Authors

Chun-Yen Chen¹, En-Wei Kuo², Dillirani Nagarajan³, Shih-Hsin Ho⁴, Cheng-Di Dong⁵, Duu-Jong Lee⁶, Jo-Shu Chang⁷

Affiliations

¹ University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
² Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
³ Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
⁴ State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, China.
⁵ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan.
⁶ Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
⁷ Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Department of Chemical and Materials Engineering, College of Engineering, Tunghai University, Taichung 407, Taiwan; Center for Nanotechnology, Tunghai University, Taichung 407, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.

PMID: 32004812
DOI: 10.1016/j.biortech.2020.122814

Abstract

Swine wastewater is rich in nitrogen and organic carbon which are essential macronutrients for microalgal growth. Three indigenous microalgal strains (Chlorella sorokiniana AK-1, Chlorella sorokiniana MS-C1, and Chlorella sorokiniana TJ5) were examined for their growth capability in untreated swine wastewater. C. sorokiniana AK-1 showed the best tolerance towards swine wastewater, and obtained the highest biomass concentration (5.45 g/L) and protein productivity (0.27 g/L/d) when grown in 50% strength swine wastewater. Cell immobilization using sponge as the solid carrier further enhanced maximal biomass concentration and protein productivity to 8.08 g/L and 0.272 g/L/d, respectively. Reuse of microalgae loaded sponge resulted in an average biomass production and protein productivity of 6.51 g/L and 0.15 g/L/d, respectively. The COD, TN and TP removal efficiency for the swine wastewater was 90.1, 97.0 and 92.8%, respectively. This innovative swine wastewater treatment method has demonstrated excellent performance on simultaneous swine wastewater treatment and protein-rich microalgal biomass production.

Keywords: Cell immobilization; Chlorella sorokiniana; Microalgal protein production; Sponge; Swine wastewater.

MeSH terms

Animals
Biomass
Chlorella*
Microalgae*
Nitrogen
Swine
Wastewater

Substances

Waste Water
Nitrogen