Five mitochondrial genomes of black fungus gnats (Sciaridae) and their phylogenetic implications

Xiaoqian Miao; Junhao Huang; Frank Menzel; Qingyun Wang; Qiaoyu Wei; Xiao-Long Lin; Hong Wu

doi:10.1016/j.ijbiomac.2020.01.271

Five mitochondrial genomes of black fungus gnats (Sciaridae) and their phylogenetic implications

Int J Biol Macromol. 2020 May 1:150:200-205. doi: 10.1016/j.ijbiomac.2020.01.271. Epub 2020 Jan 28.

Authors

Xiaoqian Miao¹, Junhao Huang², Frank Menzel³, Qingyun Wang¹, Qiaoyu Wei¹, Xiao-Long Lin⁴, Hong Wu¹

Affiliations

¹ Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, China.
² Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, China. Electronic address: huangjh@zafu.edu.cn.
³ Senckenberg Deutsches Entomologisches Institut, Eberswalder Straße 90, 15374 Müncheberg, Germany. Electronic address: frank.menzel@senckenberg.de.
⁴ College of Life Sciences, Nankai University, Tianjin 300071, China.

PMID: 32004603
DOI: 10.1016/j.ijbiomac.2020.01.271

Abstract

Sciaridae is a family of great species diversity, distributed worldwide, that includes important agricultural pests of cultivated mushrooms and plants produced in greenhouses. Here we sequenced five nearly complete mitochondrial genomes representing three subfamilies of Sciaridae. The lengths of these mitogenomes range from 13,849 bp to 16,923 bp with 13 protein-coding genes (PCGs), 20-22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region (CR). Compared with other dipteran species, rearrangements in Sciaridae are more common. Inversion or transition is observed frequently of trnL2, and in the tRNA clusters trnI-trnQ-trnM, trnW-trnC-trnY, and trnA-trnR-trnN-trnS1-trnE-trnF. Phylogenetic relationships within the family were reconstructed based on these newly sequenced species, combined with the published mitogenomes of related families, and recovered the topology within Sciaroidea as Cecidomyiidae + (Sciaridae + Keroplatidae). Relationships recovered within Sciaridae were Sciarinae + ('Pseudolycoriella group' + Megalosphyinae).

Keywords: Diptera; Molecular phylogeny; Sciariodea.

MeSH terms

Animals
Computational Biology / methods
Diptera / classification*
Diptera / genetics*
Genes, Mitochondrial*
Genome, Mitochondrial*
Genomics / methods
Molecular Sequence Annotation
Phylogeny*