Prime Editing: A New Way for Genome Editing

Abstract

Precise and efficient use of genome editing tools are hampered by the introduction of DNA double-strand breaks, donor DNA templates, or homology-directed repair. A recent study expands the genome editing toolbox with the introduction of prime editing, which overcomes previous challenges and introduces insertions, deletions, and all putative 12 types of base-to-base conversions in human cells.

Keywords: CRISPR-Cas9; genome editing; prime editing; targeted mutagenesis.