A serological point-of-care test for Zika virus detection and infection surveillance using an enzyme-free vial immunosensor with a smartphone

Ying-Pei Hsu; Nan-Si Li; Ying-Tzu Chen; Hao-Han Pang; Kuo-Chen Wei; Hung-Wei Yang

doi:10.1016/j.bios.2019.111960

A serological point-of-care test for Zika virus detection and infection surveillance using an enzyme-free vial immunosensor with a smartphone

Biosens Bioelectron. 2020 Mar 1:151:111960. doi: 10.1016/j.bios.2019.111960. Epub 2019 Dec 24.

Authors

Ying-Pei Hsu¹, Nan-Si Li², Ying-Tzu Chen³, Hao-Han Pang², Kuo-Chen Wei⁴, Hung-Wei Yang⁵

Affiliations

¹ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan; Department of Materials and Optoelectronic Science, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
² Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan; Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
³ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
⁴ Department of Neurosurgery, Chang Gung Memorial Hospital, Linkou, Taoyuan, 33305, Taiwan; School of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.
⁵ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan. Electronic address: howardyang@mail.nsysu.edu.tw.

PMID: 31999574
DOI: 10.1016/j.bios.2019.111960

Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus (FLAV) that emerged in Brazil in 2015 and has rapidly spread to more than 50 countries worldwide. However, early, accurate, and specific point-of-care (POC) diagnosis of ZIKV is very difficult because most infected patients are asymptomatic or display nonspecific symptoms similar to those of other viral infections, and most of the analysis also requires instruments. Herein, an instrument-free ZIKV POC test using a drop of blood comprising a vial immunosensor, artificial nanozyme platinum/gold core-shell nanoparticles (Pt@Au NPs) as a signal probe, and a smartphone was developed to specifically detect ZIKV without cross-reaction with other FLAVs. A high sensitivity of 1 pg/mL ZIKV, desirable specificity, data storage, and geographic location surveillance were simultaneously achieved for the proposed POC test. Our POC test suitably met the urgent needs of ports of entry, airports, and endemic regions with stressed resources, as well as strict clinical requirements for ZIKV detection.

Keywords: Nanozyme; Serological point-of-care; Smartphone reader; Vial immunosensor; Zika virus.

MeSH terms

Antibodies, Viral / genetics*
Biosensing Techniques*
Cross Reactions
Enzyme-Linked Immunosorbent Assay / methods
Humans
Point-of-Care Systems
Point-of-Care Testing
Smartphone
Zika Virus / genetics
Zika Virus / isolation & purification*
Zika Virus Infection / diagnosis*
Zika Virus Infection / virology

Substances

Antibodies, Viral