Erigeron annuus Protects PC12 Neuronal Cells from Oxidative Stress Induced by ROS-Mediated Apoptosis

Ji Yeon Lee; Jeong-Yong Park; Dong Hwi Kim; Hyung Don Kim; Yun-Jeong Ji; Kyung Hye Seo

doi:10.1155/2020/3945194

Erigeron annuus Protects PC12 Neuronal Cells from Oxidative Stress Induced by ROS-Mediated Apoptosis

Evid Based Complement Alternat Med. 2020 Jan 6:2020:3945194. doi: 10.1155/2020/3945194. eCollection 2020.

Authors

Ji Yeon Lee^{1

2}, Jeong-Yong Park^{1

2}, Dong Hwi Kim¹, Hyung Don Kim¹, Yun-Jeong Ji¹, Kyung Hye Seo¹

Affiliations

¹ Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung 27709, Republic of Korea.
² Department of Food Science and Biotechnology, Chungbuk National University, Chungbuk 28644, Republic of Korea.

Abstract

Reactive oxygen species (ROS), associated with oxidative stress, are involved in many biological processes such as apoptosis, necrosis, and autophagy. Oxidative stress might induce neuronal damage via ROS generation, causing neurodegenerative diseases. Erigeron annuus (EA) has antioxidant properties and could protect neurons from oxidative stress. In this study, we investigated the protective effect of the aerial parts (EAA) and flowers (EAF) from EA on ROS-mediated apoptosis in pheochromocytoma 12 cells. We quantified 18 types of phenolic compounds using high-performance liquid chromatography. Pretreatment of the cells with EAA and EAF attenuated ROS generation and induced the expression of antioxidant enzymes such as superoxide dismutase 2, catalase, and glutathione peroxidase. In addition, EAF reduced the expression of apoptotic proteins such as Bax/Bcl-xL, caspase-3, and caspase-8 to a greater extent than that with EAA. These results suggested that the protective effect of EAF against oxidative stress-induced apoptosis might be due to the prevention of ROS generation mediated by oxidative enzymes.