An FT-IR and XPS spectroscopy dataset of Pinus ponderosa sporopollenin and related samples to elucidate sporopollenin structural features

Alec Lutzke; Kevin J Morey; June I Medford; Matt J Kipper

doi:10.1016/j.dib.2020.105129

An FT-IR and XPS spectroscopy dataset of Pinus ponderosa sporopollenin and related samples to elucidate sporopollenin structural features

Data Brief. 2020 Jan 11:29:105129. doi: 10.1016/j.dib.2020.105129. eCollection 2020 Apr.

Authors

Alec Lutzke¹, Kevin J Morey¹, June I Medford², Matt J Kipper¹

Affiliations

¹ Department of Chemical & Biological Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
² Department of Biology, Colorado State University, Fort Collins, CO, 80523, USA.

Abstract

The ATR FT-IR spectra of Pinus ponderosa sporopollenin isolated from pollen spores by enzymatic digestion. Sporopollenin is also isolated by solvent extraction, followed by either acidolysis with phosphoric acid, and acetolysis is reported [1]. The FT-IR spectra are supplemented by XPS data of the isolated sporopollenin samples. The enzymatically isolated sporopollenin is subjected to a variety of chemical treatments and modifications, including alkaline hydrolysis, deuteration (by both D₂0 and methanol-d₄), sodium cyanoborohydride reduction, hydrolysis by peracetic acid, bromination, acetylization with acetone and octanal, and acid-catalyzed ketal cleavage. The sporopollenin isolated by acidolysis and acetolysis are also subjected to alkaline hydrolysis. The sporopollenin samples are compared to a variety of model compounds representative of putative structural constituents and functional groups.

Keywords: Biopolymer; Cellulose; Exine; Infrared spectroscopy; Pectin; Pollen; Sporopollenin; X-ray photoelectron spectroscopy.