White pepper-derived ratiometric carbon dots for highly selective detection and imaging of coenzyme A

Ruiqing Long; Ying Guo; Lianwu Xie; Shuyun Shi; Jinju Xu; Chaoying Tong; Qinlu Lin; Te Li

doi:10.1016/j.foodchem.2020.126171

White pepper-derived ratiometric carbon dots for highly selective detection and imaging of coenzyme A

Food Chem. 2020 Jun 15:315:126171. doi: 10.1016/j.foodchem.2020.126171. Epub 2020 Jan 9.

Authors

Ruiqing Long¹, Ying Guo², Lianwu Xie³, Shuyun Shi⁴, Jinju Xu¹, Chaoying Tong¹, Qinlu Lin⁵, Te Li¹

Affiliations

¹ Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
² Department of Clinical Pharmacology, Xiangya Hospital, Hunan Key Laboratory of Pharmacogenetics, Central South University, 410078 Changsha, PR China. Electronic address: guoying881212@csu.edu.cn.
³ College of Sciences, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China. Electronic address: xielianwu@csuft.edu.cn.
⁴ Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; College of Sciences, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China. Electronic address: shuyshi@gmail.com.
⁵ College of Sciences, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China. Electronic address: linql0403@126.com.

PMID: 31991253
DOI: 10.1016/j.foodchem.2020.126171

Abstract

A new-style white pepper derived dual-emission carbon dots (CDs) with a quantum yield of 10.4% was designed and facile constructed with one-pot solvothermal method. The green emission (520 nm) had an efficient and special "turn-on" fluorescence sensing of coenzyme A (CoA) with the aid of Cu²⁺, while red emission (668 nm) barely changed and worked as reference. In the concentration range (0-150 µM), relative fluorescence intensity ratios (F₅₂₀/F₆₆₈) showed excellent linear correlation with concentrations of CoA, and detection limit was as low as 8.75 nm. Moreover, the strategy has been successfully applied for label-free detection of CoA in real pig liver samples with good recoveries (93.3-108.0%). Notably, the synthesized CDs had durable fluorescence, low cytotoxicity, and good biocompatibility for cellular imaging, which demonstrated wide and promising applicability for biosensing and bioimaging in the future.

Keywords: Carbon dots; Cellular imaging; Coenzyme A; Ratiometric nanosensor; White pepper.

MeSH terms

Animals
Carbon / chemistry*
Coenzyme A / analysis*
Fluorescence
Green Chemistry Technology
HeLa Cells
Humans
Limit of Detection
Liver / chemistry
Molecular Imaging / instrumentation
Molecular Imaging / methods*
Piper nigrum / chemistry*
Quantum Dots / chemistry*
Quantum Dots / toxicity
Swine
Toxicity Tests

Substances

Carbon
Coenzyme A