Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor

Alexandra G Pershina; Olga Ya Brikunova; Natalya A Perekucha; Alexander M Demin; Oleg B Shevelev; Dina Malkeyeva; Elena Kiseleva; Artem S Minin; Larisa A Kostikova; Ivan V Stepanov; Dmitriy K Kuznetsov; Vladimir Ya Shur; Victor P Krasnov

doi:10.1016/j.dib.2019.105062

Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor

Data Brief. 2019 Dec 31:29:105062. doi: 10.1016/j.dib.2019.105062. eCollection 2020 Apr.

Affiliations

¹ Siberian State Medical University, 2, Moskovsky trakt, 634050, Tomsk, Russia.
² Research School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 30, Lenin Ave., Tomsk, 634050, Russia.
³ Postovsky Institute of Organic Synthesis UB RAS, 22, S. Kovalevskaya St., 620990, Yekaterinburg, Russia.
⁴ Institute of Cytology and Genetics SB RAS, 10, Lavrentiev Ave., 630090, Novosibirsk, Russia.
⁵ Miheev Institute of Metal Physics UB RAS, 18, S. Kovalevskaya St., 620990, Yekaterinburg, Russia.
⁶ Institute of Natural Sciences and Mathematics, Ural Federal University, 51, Lenin Ave., 620000, Yekaterinburg, Russia.

Abstract

The method of Fe₃O₄ magnetic nanoparticle synthesis by co-precipitation, modification by 3-aminopropylsilane and conjugation with pH-(low)-insertion peptide (pHLIP) is reported. The characterization of nanoparticles by scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, elemental and thermogravimetric analyses as well as dynamic light scattering and z-potential measurements is provided. The effect of nanoparticles on the viability of mouse and human peripheral blood mononuclear cells is tested by flow cytometry. The experimental details of nanoparticle administration to tumor-bearing mice, magnetic resonance imaging scanning as well as subsequent tumor sample collection and their processing for transmission electron microscopy, inductively coupled plasma atomic emission spectroscopy, histological and immunohistochemical analyses are described. Biodistribution of the nanoparticles in mice and blood serum analysis data for experimental animals are given. The data are useful for an experiment workflow design and for the development of theranostic systems based on magnetic nanoparticles.

Keywords: Biodistribution; Cytotoxicity; Iron oxide magnetic nanoparticle; Nanoparticle characterization; Tumor; pHLIP.