Establishment of nDart1-tagged lines of Koshihikari, an elite variety of rice in Japan

Hideki Nishimura; Eiko Himi; Kazuhide Rikiishi; Kazuo Tsugane; Masahiko Maekawa

doi:10.1270/jsbbs.19049

Establishment of nDart1-tagged lines of Koshihikari, an elite variety of rice in Japan

Breed Sci. 2019 Dec;69(4):696-701. doi: 10.1270/jsbbs.19049. Epub 2019 Nov 14.

Authors

Hideki Nishimura¹, Eiko Himi¹, Kazuhide Rikiishi¹, Kazuo Tsugane², Masahiko Maekawa¹

Affiliations

¹ Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
² National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan.

Abstract

To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

Keywords: DNA transposon; Koshihikari; insertional mutagenesis; nDart1-tagged lines; rice.