Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers

Hui Dong; Xiaodong Shang; Xiaoyan Zhao; Hailong Yu; Ning Jiang; Meiyan Zhang; Qi Tan; Changyan Zhou; Lujun Zhang

doi:10.1270/jsbbs.18123

Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers

Breed Sci. 2019 Dec;69(4):585-591. doi: 10.1270/jsbbs.18123. Epub 2019 Sep 19.

Authors

Hui Dong¹, Xiaodong Shang², Xiaoyan Zhao¹, Hailong Yu², Ning Jiang², Meiyan Zhang², Qi Tan², Changyan Zhou¹, Lujun Zhang²

Affiliations

¹ Institute of Agro-Food Quality Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Laboratory of Agro-Food Quality and Safety Risk Assessment at Shanghai, Shanghai, 201403, China.
² Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering, Shanghai, 201403, China.

Abstract

Genetic mapping is a basic tool for eukaryotic genomic research. It allows the localization of genes or quantitative trait loci (QTLs) and map-based cloning. In this study, we constructed a linkage map based on DNA samples from a commercial strain L808, including two parental monokaryons and 93 single spore isolates considered with segregating to 1:1:1:1 at four mating types (A₁B₁, A₁B₂, A₂B₁ and A₂B₂). Using Simple Sequence Repeats (SSR), Sequence Related Amplified Polymorphism (SRAP), Target Region Amplified Polymorphism (TRAP) molecular markers, 182 molecular markers and two mating factors were located on 11 linkage groups (LGs). The total length of the map was 948.083 centimorgan (cM), with an average marker interval distance of 4.817 cM. Only two gaps spanning more than 20 cM was observed. The probability of 20 cM, 10 cM, 5 cM genetic distance cover one marker was 99.68%, 94.36%, 76.43% in our genetic linkage map, respectively. This is the first linkage map of Lentinula edodes using SSR markers, which provides essential information for quantitative trait analyses and improvement of genome assembly.

Keywords: Lentinula edodes; SSR; genetic linkage map.