CRISPR/Cas9 to generate plant immunity against pathogen

Madiha Zaynab; Yasir Sharif; Mahpara Fatima; Muhammad Zohaib Afzal; Mehtab Muhammad Aslam; Muhammad Fahad Raza; Muhammad Anwar; Muhammad Ammar Raza; Nelam Sajjad; Xuewei Yang; Shuangfei Li

doi:10.1016/j.micpath.2020.103996

CRISPR/Cas9 to generate plant immunity against pathogen

Microb Pathog. 2020 Apr:141:103996. doi: 10.1016/j.micpath.2020.103996. Epub 2020 Jan 24.

Authors

Affiliations

¹ College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. Electronic address: madiha.zaynab14@gmail.com.
² College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
³ College of Agriculture Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
⁴ College of Life Science Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
⁵ College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
⁶ College of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
⁷ College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. Electronic address: madiha.zaynab14@szu.edu.cn.

PMID: 31988004
DOI: 10.1016/j.micpath.2020.103996

Abstract

Different types of molecular approaches have been used for improving resistance against pathogens to secure food. Efficient and advanced genome editing tool as paralleled to earlier techniques like Zinc Finger Nuclease (ZFN), transcription activator-like effector nucleases (TALENs), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). The approach of CRISPR/Cas9 has updated our abilities of genetic manipulation in many crops. The assembly of purposes that can be achieved through CRISPR/Cas9 and its related products make it a powerful system that will expose novel prospects in the complex domain of plant-pathogen interactions and will help to develop crop resistance against pathogens. CRISPR/Cas9 engineering permits DNA endonuclease guided by an RNA for a range of genome engineering applications across various eukaryotic species and provides an effective platform to create resistance against bacteria, viruses, insects, and fungi. In this review, we discuss CRISPR-Cas9 engineered crop plants resistant to specific pathogens.

Keywords: Gene editing; Immunity; Pathogen; Plant disease.

Publication types

Review

MeSH terms

Bacterial Infections
CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats
Crops, Agricultural / genetics
Disease Resistance / genetics*
Gene Editing / methods*
Mycoses
Plant Immunity / genetics
Plants, Genetically Modified
Virus Diseases