Fluorescent Nanodiamonds with Bioorthogonally Reactive Protein-Resistant Polymeric Coatings

Ivan Rehor; Hana Mackova; Sergey K Filippov; Jan Kucka; Vladimir Proks; Jitka Slegerova; Stuart Turner; Gustaaf Van Tendeloo; Miroslav Ledvina; Martin Hruby; Petr Cigler

doi:10.1002/cplu.201300339

Fluorescent Nanodiamonds with Bioorthogonally Reactive Protein-Resistant Polymeric Coatings

Chempluschem. 2014 Jan;79(1):21-24. doi: 10.1002/cplu.201300339. Epub 2013 Dec 11.

Authors

Affiliations

¹ Institute of Organic Chemistry and Biochemistry, v.v.i. The Academy of Sciences of the Czech Republic, Flemingovo nam. 2, 166 10 Prague 6 (Czech Republic), Fax: (+420) 220-183-578 www.petrcigler.cz.
² Institute of Macromolecular Chemistry, v.v.i. The Academy of Sciences of the Czech Republic, Heyrovskeho nam. 2, 162 06 Prague 6 (Czech Republic), Fax: (+420) 296-809-410.
³ Nuclear Physics Institute, v.v.i. The Academy of Sciences of the Czech Republic, 250 68 Rez near Prague (Czech Republic).
⁴ EMAT, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp (Belgium).

PMID: 31986754
DOI: 10.1002/cplu.201300339

Abstract

The novel synthesis of a polymeric interface grown from the surface of bright fluorescent nanodiamonds is reported. The polymer enables bioorthogonal attachment of various molecules by click chemistry; the particles are resistant to nonspecific protein adsorption and show outstanding colloidal stability in buffers and biological media. The coating fully preserves the unique optical properties of the nitrogen-vacancy centers that are crucial for bioimaging and sensoric applications.

Keywords: click chemistry; fluorescence; nanoparticles; polymerization; solubilization.

Abstract

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