A new aptamer/black phosphorous interdigital electrode for malachite green detection

Lidong Wu; Zhiyuan Xu; Qingyi Meng; Yushi Xiao; Qiang Cao; Brijesh Rathi; Huan Liu; Gang Han; Jing Zhang; Jiang Yan

doi:10.1016/j.aca.2019.11.026

A new aptamer/black phosphorous interdigital electrode for malachite green detection

Anal Chim Acta. 2020 Feb 22:1099:39-45. doi: 10.1016/j.aca.2019.11.026. Epub 2019 Nov 13.

Authors

Lidong Wu¹, Zhiyuan Xu², Qingyi Meng², Yushi Xiao³, Qiang Cao³, Brijesh Rathi⁴, Huan Liu⁵, Gang Han⁵, Jing Zhang², Jiang Yan²

Affiliations

¹ Key Laboratory of Control of Quality and Safety for Aquatic Products, Chinese Academy of Fishery Sciences, Beijing, 100141, China. Electronic address: lidongwu@mit.edu.
² College of Information, North China University of Technology, Beijing, 100043, China.
³ Key Laboratory of Control of Quality and Safety for Aquatic Products, Chinese Academy of Fishery Sciences, Beijing, 100141, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
⁴ Department of Chemistry, Hansraj College University of Delhi, Delhi, 110007, India.
⁵ Key Laboratory of Control of Quality and Safety for Aquatic Products, Chinese Academy of Fishery Sciences, Beijing, 100141, China.

PMID: 31986275
DOI: 10.1016/j.aca.2019.11.026

Abstract

Malachite Green (MG), a cationic triphenylmethane dye, has adverse effects on the immune and reproductive system. Thus, it is essential to develop a rapid, sensitive and high-selective method for determination of MG. Black phosphorus (BP) has high charge-carrier mobility (∼1000 cm² V^-1 s^-1) and high adsorption capacity for cationic dyes (i.e. MG) through both electrostatic and hydrophobic interactions. Thus, it potentially plays as a high-sensitive sensing platform for detecting MG. However, BP degrades within 12 h under humid condition, which limits its applications. To overcome this issue, cysteine (CYS) is used for protecting BP from oxidation and ceasing its degradation. To the best of our knowledge, it is the first time that CYS is used to functionalize BP, and a silicon interdigital electrode is fabricated with the functionalized BP and aptamer. The BP-based interdigital electrode shows a lowest detection limit of 0.3 ng L^-1 toward MG. This work provides a new route to prepare a large scale and selective biosensor for MG monitoring on site in future.

Keywords: Aptamer; Interdigital electrode; Malachite green; Portability; The CYS-BP-AuNP nanocomposite.