Aim: We aim to demonstrate that a surface-enhanced Raman spectroscopy (SERS) probe can be effectively used for protein detection in adrenal tumors. Materials & methods: The SERS probe method, which uses Au@Ag core-shell nanoparticles conjugated with a CgA antibody and a SERS reporter, was applied to detect CgA in adrenal tumors. Results: Our data reveal that the results of the CgA-SERS probe method were almost identical to those of western blot and superior to those of traditional immunohistochemistry. Conclusion: This study offers a novel strategy to detect CgA in adrenal tumors and provides more reliable protein test results than traditional immunohistochemistry analysis for adrenal pathologists, meaning that it might be a better clinical reference for the diagnosis of pheochromocytoma.
Keywords: Raman imaging; chromogranin A; immunohistochemistry; pheochromocytoma; surface-enhanced Raman scattering.