In this work, a single-layered graphene (SLG)/Au-nanoparticles (NPs)-based Love wave biosensor was prepared for detection of Staphylococcus aureus (S. aureus) gene sequences. The annealing process was proposed to obtain a larger-area interface with ssDNA. The sensitivity was verified by detecting S. aureus gene sequences with a linear detection ranging from 0 to 10 nmol/L, where the limit of detection (LOD) was only 12.4 pg/mL. The stable state of the biosensors based on SLG/Au NPs in the liquid phase could be kept for more than 0.5 h (±0.1°), and the specificity was verified by detecting noncomplementary ssDNA and one- and three-base mismatched ssDNA. The results of our study suggest that a Love wave biosensor based on SLG/Au NPs hold great potential in clinical testing and diagnosis.
Keywords: SLG/Au NPs; Staphylococcus aureus gene sequence; biosensor; chemical vapor deposition; electron beam evaporation.