Genomic Characterization of Tilapia Lake Virus Isolates Recovered from Moribund Nile Tilapia (Oreochromis niloticus) on a Farm in the United States

Mohammad Shamim Ahasan; William Keleher; Cem Giray; Brenda Perry; Win Surachetpong; Pamela Nicholson; Lowia Al-Hussinee; Kuttichantran Subramaniam; Thomas B Waltzek

doi:10.1128/MRA.01368-19

Genomic Characterization of Tilapia Lake Virus Isolates Recovered from Moribund Nile Tilapia (Oreochromis niloticus) on a Farm in the United States

Microbiol Resour Announc. 2020 Jan 23;9(4):e01368-19. doi: 10.1128/MRA.01368-19.

Authors

Mohammad Shamim Ahasan^{1

2

3}, William Keleher⁴, Cem Giray⁴, Brenda Perry⁴, Win Surachetpong^{5

6}, Pamela Nicholson⁷, Lowia Al-Hussinee^{1

2}, Kuttichantran Subramaniam^{1

2}, Thomas B Waltzek^{8

2}

Affiliations

¹ Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
² Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA.
³ Faculty of Veterinary and Animal Sciences, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Rangpur, Bangladesh.
⁴ Kennebec River Biosciences, Richmond, Maine, USA.
⁵ Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.
⁶ Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
⁷ Next Generation Sequencing Platform, University of Bern, Bern, Switzerland.
⁸ Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA tbwaltzek@ufl.edu.

Abstract

Here, we present the complete coding sequences of two tilapia lake virus (TiLV) isolates recovered during an investigation of a mortality event in farmed Nile tilapia in the United States. Phylogenetic analysis supported the isolates as each other's closest relatives and members of a clade of Thai TiLV strains.