Antiangiogenic activity of terpenoids from Euphorbia neriifolia Linn

Bioorg Chem. 2020 Mar:96:103536. doi: 10.1016/j.bioorg.2019.103536. Epub 2019 Dec 28.

Abstract

Angiogenesis is known to serve an important role in embryonic development, wound healing, tissue regeneration, and growth. Two new abietane-type diterpenoids (3, 5), a new lanosterol triterpenoid (8) and seven known compounds haven been isolated from the Euphorbia neriifolia Linn. The structures of all compounds were elucidated by spectroscopic analysis and comparing their NMR data with reported data. Furthermore, we found that compounds 6 and 9 had the antiangiogenic effects in vitro. They could inhibit HUVEC migration and microvessel sprouting in rat aortic rings. Moreover, compound 6 inhibited VEGFR and phosphorylation of Akt, but compound 9 only shown inhibitory effect on phosphorylation of Akt. Taken together, these results suggest that inhibition of VEGF signaling and downstream pathways may be responsible for the antiangiogenic activity of compounds 6 and 9.

Keywords: Anti-angiogenesis; Euphorbia neriifolia; Migration; Rat aortic rings; Terpenoid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / isolation & purification
  • Angiogenesis Inhibitors / pharmacology*
  • Animals
  • Aorta / drug effects
  • Cell Movement / drug effects
  • Euphorbia / chemistry*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • In Vitro Techniques
  • Proto-Oncogene Proteins c-akt / antagonists & inhibitors
  • Proto-Oncogene Proteins c-akt / metabolism
  • Rats
  • Rats, Wistar
  • Receptors, Vascular Endothelial Growth Factor / antagonists & inhibitors
  • Receptors, Vascular Endothelial Growth Factor / metabolism
  • Signal Transduction / drug effects
  • Terpenes / isolation & purification
  • Terpenes / pharmacology*

Substances

  • Angiogenesis Inhibitors
  • Terpenes
  • Receptors, Vascular Endothelial Growth Factor
  • Proto-Oncogene Proteins c-akt