Data on enrichment of chitosan nanoparticles for intranasal delivery of oligonucleotides to the brain

Vasyl Sava; Oksana Fihurka; Anastasia Khvorova; Juan Sanchez-Ramos

doi:10.1016/j.dib.2019.105093

Data on enrichment of chitosan nanoparticles for intranasal delivery of oligonucleotides to the brain

Data Brief. 2020 Jan 3:28:105093. doi: 10.1016/j.dib.2019.105093. eCollection 2020 Feb.

Authors

Vasyl Sava¹, Oksana Fihurka¹, Anastasia Khvorova², Juan Sanchez-Ramos¹

Affiliations

¹ Department of Neurology, University of South Florida, Tampa, FL, USA.
² RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA.

Abstract

Data on preparation and characterization of chitosan-based nanoparticles (NP) carrying small interfering RNA (siRNA) for non-invasive gene therapy is presented. Polyelectrolyte complexation method was carried out in diluted concentrations to obtain relatively small (less than 200 nm) NP. To provide substantial dose of siRNA within tolerable volume of intranasal administration the NP were subjected to enrichment process. Offered here NP fabrication does two steps process comprise provisional and enriched preparations? The differences between these preparations were analyzed with hydrodynamic size distribution and zeta potential measurements. The effect of siRNA lipophilicity on NP physical instability was also tested. Biological evaluation of nanoparticles is described in our published article [1].

Keywords: Chitosan; Enrichment; Nanocarriers; Polyelectrolyte complexation; siRNA.

Grants and funding

S10 OD020012/OD/NIH HHS/United States