Biodegradation of Deoxynivalenol by a Novel Microbial Consortium

Yanxia Wang; Gang Wang; Yijun Dai; Yu Wang; Yin-Won Lee; Jianrong Shi; Jianhong Xu

doi:10.3389/fmicb.2019.02964

Biodegradation of Deoxynivalenol by a Novel Microbial Consortium

Front Microbiol. 2020 Jan 8:10:2964. doi: 10.3389/fmicb.2019.02964. eCollection 2019.

Authors

Yanxia Wang^{1

2}, Gang Wang², Yijun Dai¹, Yu Wang^{1

2}, Yin-Won Lee^{2

3}, Jianrong Shi^{2

4}, Jianhong Xu^{2

4}

Affiliations

¹ Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, Nanjing, China.
² Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Key Laboratory for Agro-Product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Modern Grain Circulation and Safety, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
³ Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
⁴ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.

Abstract

Deoxynivalenol (DON), a common mycotoxin of type B trichothecene, is produced mainly by several Fusarium species. DON causes great losses in farming and poses severe safety risks to human and animal health. Thus, DON contamination in cereals and DON toxicity are of worldwide concern. In this study, we screened the bacterial consortium C20, which efficiently degraded almost 70 μg ml^-1 DON within 5 days. The bacterial consortium also had the ability to degrade 15-acetyl-DON, 3-acetyl-DON, and T-2 toxin. The bacterial consortium C20 was able to degrade DON under a wide range of pH and temperature conditions. The optimal temperature and pH for DON degradation were 30°C and pH 8.0, respectively. The bacterial consortium C20 comprised of different bacterial genera, and several strains were found to significantly increase when cultured in Mineral Medium with 100 μg ml^-1 DON based on the analysis of the sequences of the hypervariable V3-V4 region of the 16S rRNA gene. 3-keto-DON was confirmed as a degradation product of DON by liquid chromatography/time-of-flight/mass spectrometry (LC-TOF-MS) and nuclear magnetic resonance (NMR) analyses. The results indicated that the bacterial consortium C20 is a potential candidate for the biodegradation of DON in a safe and environmentally friendly manner.

Keywords: 3-keto-DON; bacterial consortium; biodegradation; deoxynivalenol; mycotoxin.