A new effective oxidative solution for titanium (Ti) surface etching was recently developed. The present in vitro study was aimed at determining the influence of shorter (than 240 min) treatment time on the surface characteristics of the Ti nano/micro hierarchical structures. Cylinder-shaped Ti grade 5 alloys were etched for 30, 60, 120, and 240 min at room temperature and cleaned successively with acetone, ethanol, and distilled water in an ultrasonic bath. The micro- and nanostructures, surface roughness, dynamic wettability, and the surface elemental composition of the etched surfaces were evaluated. Nano/micro hierarchical structures, composed of micro-pits and nano-channels, were formed on the Ti surface through simple immersion in the oxidative solution. The findings suggest that the 120-min immersion yielded significant enhancement in the roughness and wettability of the Ti surfaces.