Rapid high resolution melting assay to differentiate Streptococcus suis serotypes 2, 1/2, 1, and 14

Simone Scherrer; Fenja Rademacher; Nathalie Spoerry Serrano; Jacques Schrenzel; Marcelo Gottschalk; Roger Stephan; Patricia Landolt

doi:10.1002/mbo3.995

Rapid high resolution melting assay to differentiate Streptococcus suis serotypes 2, 1/2, 1, and 14

Microbiologyopen. 2020 Apr;9(4):e995. doi: 10.1002/mbo3.995. Epub 2020 Jan 22.

Authors

Simone Scherrer¹, Fenja Rademacher¹, Nathalie Spoerry Serrano¹, Jacques Schrenzel², Marcelo Gottschalk³, Roger Stephan¹, Patricia Landolt¹

Affiliations

¹ Department of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
² Bacteriology Laboratory, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
³ Swine and Poultry Infectious Diseases Research Center (CRIPA), Groupe de recherche sur les maladies infectieuses des animaux de production, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada.

Abstract

This rapid high resolution melting (HRM) assay allows distinguishing between Streptococcus suis serotype pairs 2 and 1/2 as well as 1 and 14, respectively, based on a single-nucleotide polymorphism within capsular polysaccharide synthesis gene cluster K. This assay is easy to implement and identifies potential zoonotic serotypes.

Keywords: Streptococcus suis; 1 and 14; 1/2; differentiation serotypes 2; high resolution melting.

MeSH terms

Animals
Bacterial Capsules / genetics*
Genome, Bacterial / genetics
Humans
Molecular Typing / methods*
Polymorphism, Single Nucleotide / genetics
Polysaccharides, Bacterial / genetics*
Serogroup
Serotyping / methods*
Streptococcal Infections / microbiology
Streptococcus suis / classification*
Streptococcus suis / genetics*
Swine
Swine Diseases / microbiology
Whole Genome Sequencing / methods

Substances

Polysaccharides, Bacterial