Novel Reporter System Monitoring IL-18 Specific Signaling Can Be Applied to High-Throughput Screening

Mar Drugs. 2020 Jan 17;18(1):60. doi: 10.3390/md18010060.

Abstract

Very recently, the immunotherapies against cancer, autoimmune diseases, and infection have been feasible and promising. Thus, we have examined the possibility whether or not human gamma delta T cells can be applied for the novel immunotherapies. We previously established the cells stably maintaining NFkB-driven human secreted embryonic alkaline phosphatase (SEAP) expression. The cells can be used to determine the transcription activity of NFkB with high-standard dynamic range and accuracy. Because IL-18 is a kind of cytokines that enhances cytotoxicity and activity of human gamma delta T cells through NFkB activation, we have focused on the activity and signaling of IL-18. In this study, we modified the previous reporter cell that can determine the transcription activity of NFkB to express two subunits consisted of human IL-18 receptor. The modified cells secreted SEAP in response to treatment with human recombinant IL-18 in a concentration-dependent manner. We also observed the concentration-dependently enhancement of NFkB activity in the cells treated with mouse recombinant IL-18 although the affinity was lower compared to human recombinant IL-18. We also previously established the cells stably expressing and secreting human recombinant IL-18 and then validated whether or not the conditioned medium from the cells activate NFkB transcription activity using this assay. Our university has kept collecting many extracts from over 18,000 marine bacteria in our local sea around Omura bay-fungi, plants for Chinese herbal medicine, and so on-and also have kept gathering synthetic compounds from many Japanese chemists as drug libraries. Finally, in order to identify drugs mimicking IL-18 biological activity or possessing inhibitory effects on IL-18-induced NFkB, we demonstrated drug screening using number of extracts derived from marine bacteria and synthetic compounds.

Keywords: EBNA1; IL-18R1; IL-18Rap; drug screening; marine bacteria; pNFkB-SEAP; synthetic compound.

MeSH terms

  • Aquatic Organisms / metabolism
  • Bacteria / metabolism
  • Biological Assay / methods
  • Drug Evaluation, Preclinical / methods
  • High-Throughput Screening Assays / methods
  • Humans
  • Interleukin-18 / metabolism*
  • NF-kappa B / metabolism
  • Signal Transduction / physiology*

Substances

  • IL18 protein, human
  • Interleukin-18
  • NF-kappa B