Generation of a human control iPS cell line (ESi080-A) from a donor with no rheumatic diseases

Stem Cell Res. 2020 Mar:43:101683. doi: 10.1016/j.scr.2019.101683. Epub 2019 Dec 9.

Abstract

Here, we report the establishment of the human iPS cell line N1-FiPS4F#7 generated from skin cells of a patient with no rheumatic diseases, thus obtaining an appropriate control iPS cell line for researchers working in the field of rheumatic diseases. The reprogramming factors Oct4, Sox2, Klf4 and c-Myc were introduced using a non-integrating reprogramming strategy involving Sendai Virus.

Keywords: Cell line; Embryoid body; Human iPS; Pluripotency; Reprogramming; Sendai virus.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming / genetics*
  • Female
  • Humans
  • Induced Pluripotent Stem Cells / metabolism*
  • Kruppel-Like Factor 4
  • Rheumatic Diseases / genetics*
  • Tissue Donors