Influence of N-acylhomoserine lactonase silver nanoparticles on the quorum sensing system of Helicobacter pylori: A potential strategy to combat biofilm formation

Vinoj Gopalakrishnan; Esakkirajan Masanam; Vijayan S Ramkumar; Vaseeharan Baskaraligam; Gopinath Selvaraj

doi:10.1002/jobm.201900537

Influence of N-acylhomoserine lactonase silver nanoparticles on the quorum sensing system of Helicobacter pylori: A potential strategy to combat biofilm formation

J Basic Microbiol. 2020 Mar;60(3):207-215. doi: 10.1002/jobm.201900537. Epub 2020 Jan 21.

Authors

Vinoj Gopalakrishnan^{1

2}, Esakkirajan Masanam³, Vijayan S Ramkumar⁴, Vaseeharan Baskaraligam³, Gopinath Selvaraj⁵

Affiliations

¹ Central Inter-Disciplinary Research Facility, Mahatma Gandhi Medical College and Hospital, Sri Balaji Vidyapeeth University, Pondicherry, India.
² Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Rehovot, Israel.
³ Department of Animal Health and Management, Alagappa University, Karaikudi, India.
⁴ Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
⁵ Department of Microbiology, Agricultural Research Organization, Newe Ya'ar, Israel.

PMID: 31960983
DOI: 10.1002/jobm.201900537

Abstract

The treatment of Helicobacter pylori usually fails due to their ability to form biofilms and resistance to antibiotics. This might potentially lead to gastric carcinoma and mucosa-associated lymphoid tissue lymphoma. In the present study, we elucidate the potential role of N-acylhomoserine lactonase stabilized silver nanoparticles (AiiA-AgNPs) in treating biofilms produced by H. pylori. AiiA-AgNPs inhibited quorum sensing (QS) by degradation of QS molecules, thereby reducing biofilm formation, urease production, and altering cell surface hydrophobicity of H. pylori. AiiA-AgNPs showed no cytotoxic effects on RAW 264.7 macrophages at the effective concentration (1-5 µM) of antibiofilm activity. In addition, AiiA-AgNP in high concentration (80-100 µM) exhibited cytotoxicity against HCT-15 carcinoma cells, depicting its therapeutic role in treating cancer.

Keywords: AiiA-AgNPs; Helicobacter pylori; biofilm; cytotoxicity; quorum sensing.

MeSH terms

Animals
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology
Biofilms / drug effects*
Biofilms / growth & development
Carboxylic Ester Hydrolases / chemistry
Carboxylic Ester Hydrolases / pharmacology*
Cell Line, Tumor
Cell Membrane / chemistry
Cell Membrane / drug effects
Cell Survival / drug effects
Helicobacter pylori / drug effects*
Helicobacter pylori / metabolism
Humans
Hydrophobic and Hydrophilic Interactions / drug effects
Metal Nanoparticles / chemistry
Mice
Quorum Sensing / drug effects*
RAW 264.7 Cells
Silver / chemistry
Silver / pharmacology*
Urease / metabolism

Substances

Anti-Bacterial Agents
Antineoplastic Agents
Silver
Carboxylic Ester Hydrolases
N-acyl homoserine lactonase
Urease