Abstract
Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA ligands was demonstrated using BePI and doxorubicin. Identification of triplex-interacting ligands may allow the regulation of genetic instability in human genomes.
MeSH terms
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Berberine Alkaloids / chemistry
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DNA / analysis*
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DNA / chemistry
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Doxorubicin / chemistry
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Genome, Human
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Genomic Instability
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Humans
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Indoles / chemistry
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Intercalating Agents / chemistry*
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Ligands
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Proto-Oncogene Proteins c-myc / genetics
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Pyridines / chemistry
Substances
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Berberine Alkaloids
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Indoles
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Intercalating Agents
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Ligands
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Proto-Oncogene Proteins c-myc
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Pyridines
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triplex DNA
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3-methoxy-7H-8-methyl-11-((3'-amino)propylamino)benzo(e)pyrido(4,3-b)indole
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coralyne
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Doxorubicin
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DNA