Carboxymethylpachymaran-zein coated plant microcapsules-based β-galactosidase encapsulation system for long-term effective delivery

Ziyu Deng; Shishuai Wang; Bin Zhou; Jing Li; Peiyuan Zhou; Bin Li; Hongshan Liang

doi:10.1016/j.foodres.2019.108867

Carboxymethylpachymaran-zein coated plant microcapsules-based β-galactosidase encapsulation system for long-term effective delivery

Food Res Int. 2020 Feb:128:108867. doi: 10.1016/j.foodres.2019.108867. Epub 2019 Dec 2.

Authors

Ziyu Deng¹, Shishuai Wang², Bin Zhou³, Jing Li¹, Peiyuan Zhou¹, Bin Li⁴, Hongshan Liang⁵

Affiliations

¹ College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
² College of Culinary and Food Engineering, Wuhan Business University, Wuhan 430056, China.
³ School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, China.
⁴ College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China; Functional Food Engineering & Technology Research Center of Hubei Province, China.
⁵ College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China. Electronic address: lianghongshan@mail.hzau.edu.cn.

PMID: 31955776
DOI: 10.1016/j.foodres.2019.108867

Abstract

Oral delivery of β-galactosidase (β-Gal) for alleviating lactose intolerance is a challenge due to its degradation in the harsh gastrointestinal tract (GIT). In this study, sunflower sporopollenin exine capsules (SECs)-based systems were fabricated to serve as protective microcapsules in order to improve the stability as well as to impact the pH-responsive release of β-Gal in GIT. The SECs extraction and loading process were optimized with the maximum residual activity of 82.75 ± 2.16%. Furthermore, the comparison of two systems indicated that β-Gal loaded into SECs which were entrapped in CMP-zein system was superior to that in zein system in terms of delivering β-Gal to the intestinal tract. Additionally, the interaction between CMP and zein confirmed by FTIR might contribute to the increased resistance to the GIT. Collectively, these results suggested that SECs-based CMP-zein system might be useful for encapsulation, protection, and delivery of bioactive substances.

Keywords: Carboxymethylpachymaran; Controlled release; Enzyme activity; Stabilization; Sunflower sporopollenin exine capsules; Zein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Capsules
Delayed-Action Preparations
Glucans / chemistry*
Microscopy, Electron, Scanning
Surface Properties
Zein / chemistry*
beta-Galactosidase / chemistry*
beta-Galactosidase / metabolism*

Substances

Capsules
Delayed-Action Preparations
Glucans
carboxymethylpachymaran
Zein
beta-Galactosidase