Chungkookjang, a soy food, fermented with Bacillus amyloliquefaciens protects gerbils against ishcmeic stroke injury, and post-stroke hyperglycemia

Do Yeon Jeong; Seong-Yeop Jeong; Ting Zhang; Xuangao Wu; Jing Yi Qiu; Sunmin Park

doi:10.1016/j.foodres.2019.108769

Chungkookjang, a soy food, fermented with Bacillus amyloliquefaciens protects gerbils against ishcmeic stroke injury, and post-stroke hyperglycemia

Food Res Int. 2020 Feb:128:108769. doi: 10.1016/j.foodres.2019.108769. Epub 2019 Oct 31.

Authors

Do Yeon Jeong¹, Seong-Yeop Jeong¹, Ting Zhang², Xuangao Wu², Jing Yi Qiu², Sunmin Park³

Affiliations

¹ Dept. of R & D, Microbial Institute for Fermentation Industry, Sunchang 56048, South Korea.
² Dept. of Food and Nutrition, Obesity/Diabetes Research Center, Hoseo University, Asan 31499, South Korea.
³ Dept. of Food and Nutrition, Obesity/Diabetes Research Center, Hoseo University, Asan 31499, South Korea. Electronic address: smpark@hoseo.edu.

PMID: 31955739
DOI: 10.1016/j.foodres.2019.108769

Abstract

Chungkookjang is a traditional Korean fermented soybean food with anti-diabetic and thrombolytic activity. It may also have anti-stroke activity. We determined that chungkookjang made with Sunchang Research Center for Fermentation Microbes 100730 and 100731 strains of Bacillus amyloliquefaciens(SRCM100730; CKJ730) and SRCM100731(CKJ731) protected against ischemic stroke and post-stroke hyperglycemia in Mongolian gerbils with ischemic stroke induced by transient occlusion of the carotid arteries. Gerbil fed 4.5% cooked soybeans (CSB), CKJ730, CKJ731, or cellulose (negative-control) in 40 energy% fat diets for 3 weeks, and then had artery occlusion for 8 min and continued taking the assigned diet for 5 weeks. CKJ730 and CKJ731 had thrombolytic activity and prevented neuronal cell death. Consequently, they improved short-term memory and spontaneous alteration compared to the negative-control. CKJ730 and CKJ731 improved neurological symptoms including drooped eyes, crouched posture, flexor reflex, and walking patterns the most among the stroke-induced gerbils. CKJ730 and CKJ731 increased active time, grip strength, and blood flow measured by Doppler compared to the negative-control. CKJ730 protected against post-stroke glucose dysregulation by restoring β-cell mass in the gerbils with transient artery occlusion. Serum tumor necrosis factor-α and interleukin-1β levels were lower in CKJ730 and CKJ731 than the negative-control. CSB also improved glucose metabolism and suppressed inflammatory cytokines, but less than CKJ730 and CKJ731. Clostridia increased, and Bacteriodia slightly decreased in the negative-control group, compared to the normal-control. CKJ730 and CKJ731 changed the amounts of Bacteriodia and Clostridia to be similar to normal-control. In conclusion, the daily intake of chungkookjang fermented with B. amyloliquefaciens improved the gut microbiome, increased blood flow to the brain, suppressed systemic inflammation, and may reduce the susceptibility to injury from ischemic stroke in gerbils subjected to ischemic injury.

Keywords: Bacillus amyloliquefaciens; Blood flow; Chungkookjang; Gerbils; Gut microbiome; β-cell mass.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacillus amyloliquefaciens
Brain Ischemia / prevention & control
Disease Models, Animal
Fermentation
Gerbillinae
Hyperglycemia / prevention & control*
Isoflavones / pharmacology*
Soy Foods
Soybean Proteins / pharmacology*
Stroke / prevention & control*

Substances

Isoflavones
Soybean Proteins
chungkookjang