Impact of wastewater-borne nanoparticles of silver and titanium dioxide on the swimming behaviour and biochemical markers of Daphnia magna: An integrated approach

Abstract

Due to their widespread use, silver (Ag) and titanium dioxide (TiO₂) nanoparticles (NPs) are commonly discharged into aquatic environments via wastewater treatment plants. The study was aimed to assess the effects of wastewater-borne AgNPs (NM-300 K; 15.5 ± 2.4 nm; 25-125 μg L^-1) and TiO₂NPs (NM-105; 23.1 ± 6.2 nm; 12.5-100 μg L^-1), from a laboratory-scale wastewater treatment plant, on Daphnia magna, at individual and subcellular level. For effect comparison, animals were also exposed to ASTM-dispersed NPs at the same nominal concentrations. The behaviour of D. magna was evaluated through monitoring of swimming height and allocation time for preferred zones after 0 h and 96 h of exposure. Biochemical markers of neurotransmission, anaerobic metabolism, biotransformation, and oxidative stress were subsequently determined. No 96-h EC₅₀ (immobilization ≤ 4 %) could be obtained with wastewater-borne NPs and ASTM-dispersed TiO₂NPs, whereas the ASTM-dispersed AgNPs resulted in an immobilization 96-h EC₅₀ of 113.8 μg L^-1. However, both wastewater-borne and ASTM-dispersed TiO₂NPs, at 12.5 μg L^-1, caused immediate (0 h) alterations on the swimming height. Allocation time analyses showed that animals exposed to ASTM-dispersed AgNPs spent more time on the surface and bottom at 0 h, and in the middle and bottom at 96 h. This pattern was not observed with ASTM-dispersed TiO₂NPs nor with wastewater-borne AgNPs and wastewater-borne TiO₂NPs. At the biochemical level, the more pronounced effects were observed with wastewater-borne AgNPs (e.g. induction of lactate dehydrogenase and glutathione S-transferase activities, and inhibition of catalase activity). This integrative approach showed that: (i) the behavioural and biochemical response-patterns were distinct in D. magna exposed to environmentally relevant concentrations of wastewater-borne and ASTM-dispersed NPs; (ii) the most pronounced effects on allocation time were induced by ASTM-dispersed AgNPs; and (iii) at the subcellular level, wastewater-borne AgNPs were more toxic than wastewater-borne TiO₂NPs. This study highlights the need for the assessment of the effects of wastewater-borne NPs under realistic exposure scenarios, since processes in wastewater treatment plants may influence their toxicity.

Keywords: AgNPs; Cladocera; Oxidative stress; TiO(2)NPs; Vertical migration; Wastewater treatment plants.