Wound dressing from polyvinyl alcohol/chitosan electrospun fiber membrane loaded with OH-CATH30 nanoparticles

Pengfei Zou; Wen-Hui Lee; Zhiqin Gao; Di Qin; Yuxia Wang; Jiao Liu; Tongyi Sun; Yuanyuan Gao

doi:10.1016/j.carbpol.2019.115786

Wound dressing from polyvinyl alcohol/chitosan electrospun fiber membrane loaded with OH-CATH30 nanoparticles

Carbohydr Polym. 2020 Mar 15:232:115786. doi: 10.1016/j.carbpol.2019.115786. Epub 2019 Dec 28.

Authors

Pengfei Zou¹, Wen-Hui Lee², Zhiqin Gao³, Di Qin³, Yuxia Wang¹, Jiao Liu¹, Tongyi Sun⁴, Yuanyuan Gao⁵

Affiliations

¹ School of Pharmacy, Weifang Medical University, Weifang, 261053, Shandong, China.
² Key Laboratory of Bioactive Peptide of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China; Key Laboratory of Biological Medicine in Universities of Shandong Province, School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, Shandong, China.
³ Key Laboratory of Biological Medicine in Universities of Shandong Province, School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, Shandong, China.
⁴ Key Laboratory of Biological Medicine in Universities of Shandong Province, School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, Shandong, China. Electronic address: sd_sty@126.com.
⁵ School of Pharmacy, Weifang Medical University, Weifang, 261053, Shandong, China. Electronic address: yyg20062006@126.com.

PMID: 31952594
DOI: 10.1016/j.carbpol.2019.115786

Abstract

Novel nanomaterials have been developed for antimicrobial and wound healing applications. Here, we report the preparation of a polyvinyl alcohol/chitosan (PVA/CS) nanofiber with carboxymethyl chitosan nanoparticles (CMCS-OH30 NPs) encapsulating the antibacterial peptide OH-CATH30 (OH-30). The PVA/CS nanofibers containing OH-30 NPs (NP-30-NFs) obtained via electrospinning could achieve a secondary embedded OH-30. The effect of NP-30-NFs on the release of OH-30 was investigated through high-performance liquid chromatography. The antibacterial activities of NP-30-NFs against Escherichia coli and Staphylococcus aureus were studied by bacterial plate counting. NP-30-NFs containing different concentrations of NPs were applied to mouse skin wounds to determine their effectiveness in promoting wound healing. Results showed that NP-30-NFs exhibited antibacterial properties and promoted skin wound healing.

Keywords: Nanofibers; Nanoparticles; OH-CATH30; PVA; Wound healing.

MeSH terms

Animals
Anti-Bacterial Agents / administration & dosage
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Antimicrobial Cationic Peptides / administration & dosage
Antimicrobial Cationic Peptides / chemistry
Antimicrobial Cationic Peptides / pharmacology*
Chitosan / administration & dosage
Chitosan / chemistry
Chitosan / pharmacology*
Dose-Response Relationship, Drug
Escherichia coli / drug effects
Female
Injections, Intraperitoneal
Mice
Mice, Inbred Strains
Microbial Sensitivity Tests
Nanofibers / administration & dosage
Nanofibers / chemistry*
Nanoparticles / administration & dosage
Nanoparticles / chemistry
Particle Size
Polyvinyl Alcohol / administration & dosage
Polyvinyl Alcohol / chemistry
Polyvinyl Alcohol / pharmacology*
Staphylococcus aureus / drug effects
Structure-Activity Relationship
Surface Properties
Wound Healing / drug effects*

Substances

Anti-Bacterial Agents
Antimicrobial Cationic Peptides
Polyvinyl Alcohol
Chitosan