Intermedin promotes hepatic carcinoma cell proliferation through upregulation of miR-155

Hai Shang; Zhi Qiang Hao; Xi Bo Fu; Xiang Dong Hua; Zuo Hong Ma; Fu Lu Ai; Zhao Qiang Feng; Kun Wang; Wen Xin Li; Bo Li

Intermedin promotes hepatic carcinoma cell proliferation through upregulation of miR-155

Int J Clin Exp Pathol. 2018 Aug 1;11(8):3961-3968. eCollection 2018.

Authors

Hai Shang¹, Zhi Qiang Hao¹, Xi Bo Fu¹, Xiang Dong Hua¹, Zuo Hong Ma¹, Fu Lu Ai¹, Zhao Qiang Feng¹, Kun Wang¹, Wen Xin Li¹, Bo Li¹

Affiliation

¹ Department of Hepatobiliary Surgery, Liaoning Cancer Hospital & Institute Shenyang 110042, Liaoning Province, China.

PMID: 31949784
PMCID: PMC6962807

Abstract

Objective: MicroRNAs (miRNAs) plays an important role in the development of malignant carcinoma. The small peptide intermedin (IMD) can promote hepatic carcinoma cell proliferation. The aim of the present study is to examine the effect of miR-155 on IMD-stimulated hepatic carcinoma cell proliferation.

Methods: Proliferation of hepatic carcinoma SMMC7721 cells was detected by CCK-8, expression of proliferating cell nuclear antigen (PCNA) and miR-155 was detected by real-time PCR.

Results: We found that IMD promotes the proliferation of SMMC7721 cells in a time and dose-dependent manner. IMD can upregulate the expression of miR-155, and blocking of miR-155 can inhibit the IMD-induced SMMC7721 cell proliferation to some extent.

Conclusion: This study demonstrated that IMD can promote the proliferation of human hepatic carcinoma cell line SMMC7721 cells through upregulation of miR-155. This study may contribute to hepatic cancer prevention and therapy.

Keywords: Intermedin; hepatic carcinoma; miR-155; proliferation.