CFSE dilution to study human T and NK cell proliferation in vitro

Iñigo Terrén; Ane Orrantia; Joana Vitallé; Olatz Zenarruzabeitia; Francisco Borrego

doi:10.1016/bs.mie.2019.05.020

CFSE dilution to study human T and NK cell proliferation in vitro

Methods Enzymol. 2020:631:239-255. doi: 10.1016/bs.mie.2019.05.020. Epub 2019 May 29.

Authors

Iñigo Terrén¹, Ane Orrantia¹, Joana Vitallé¹, Olatz Zenarruzabeitia¹, Francisco Borrego²

Affiliations

¹ Biocruces Bizkaia Health Research Institute, Immunopathology Group, Barakaldo, Spain.
² Biocruces Bizkaia Health Research Institute, Immunopathology Group, Barakaldo, Spain; Ikerbasque, Basque Foundation for Science, Bilbao, Spain. Electronic address: francisco.borregorabasco@osakidetza.eus.

PMID: 31948550
DOI: 10.1016/bs.mie.2019.05.020

Abstract

Lymphocytes proliferate in response to several stimuli. In many situations, a rapid lymphocyte expansion, or the identification of a slow dividing cell subpopulation may be of great interest. Thus, it is necessary to perform reliable assays to study and compare lymphocyte subsets proliferation. For this purpose, carboxifluorescein diacetate succinimidyl ester (CFSE) dilution assay has been stablished as a very useful tool that provides cumulative information about cell proliferation. Unlike other techniques that measure a static parameter of a specific time-point, CFSE staining allows to distinguish between subsequent cell divisions. Here, we show a simple protocol to study human T and NK cell proliferation with CFSE dilution assay by flow cytometry.

Keywords: CFSE; Cell division; IL-12; IL-15; IL-18; IL-2; Lymphocyte; NK cell; Proliferation; T cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Proliferation*
Flow Cytometry / methods*
Fluoresceins
Humans
Killer Cells, Natural / physiology*
Succinimides
T-Lymphocytes / physiology*

Substances

5-(6)-carboxyfluorescein diacetate succinimidyl ester
Fluoresceins
Succinimides