Non-melanoma skin cancers are the most common tumor in the Caucasian population, and include actinic keratosis (AK), which is considered an early form of in-situ squamous cell carcinoma (SCC). Currently the only way to monitor lesion progression (i.e., from AK to invasive SCC) is through an invasive bioptic procedure. Near-infrared spectroscopy (NIRS) is a non-invasive technique that studies haemoglobin (oxygenated haemoglobin, O2Hb, and deoxygenated haemoglobin, HHb) relative concentration variations. The objective of this study is to evaluate if AKs present a different vascular response when compared to healthy skin using time and frequency parameters extracted from the NIRS signals. The NIRS signals were acquired on the AKs and a healthy skin area of patients (n=53), with the same acquisition protocol: baseline signals (1.5 min), application of ice pack near lesion (1.5 min), removal of ice pack and acquisition of vascular recovery (1.5 min). We calculated 18 features to evaluate if the vascular response was different in the two cases (i.e., healthy skin and AK lesions). By applying the multivariate analysis of variance (MANOVA), a statistically significant difference is found in the O2Hb and HHb after the stimulus application. This shows how the NIRS technique can give important vascular information that could help the diagnosis of a lesion and the evaluation of its progression. Overall, the obtained results encourage us to look further into the study of the skin lesions and their progression with NIRS signals.