Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α0-thalassemia (SEA deletion)

Chuti Laowtammathron; Pimjai Chingsuwanrote; Roungsin Choavaratana; Suphadtra Phornwilardsiri; Ketsara Sitthirit; Chidchanok Kaewjunun; Orawan Makemaharn; Papussorn Terbto; Supaporn Waeteekul; Chanchao Lorthongpanich; Yaowalak U-Pratya; Pimonwan Srisook; Pakpoom Kheolamai; Surapol Issaragrisil

doi:10.1016/j.scr.2019.101695

Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α⁰-thalassemia (SEA deletion)

Stem Cell Res. 2020 Mar:43:101695. doi: 10.1016/j.scr.2019.101695. Epub 2020 Jan 7.

Authors

Affiliations

¹ Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address: chuti.lao@mahidol.ac.th.
² Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
³ Division of Infertility and Reproductive Biology, Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁴ Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁵ Division of Medical Genetics, Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁶ Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁷ Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Division of Cell Biology, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand.

PMID: 31945613
DOI: 10.1016/j.scr.2019.101695

Abstract

MUSIe001-A cell line was derived from a Southeast Asian (SEA) type deletion α⁰-thalassemia embryo. The SEA deletion embryo was donated for research with informed consent. This cell line shows normal hESC morphology, expresses all pluripotent markers, and has the potential to differentiate into all three germ layers in vitro and in vivo. The MUSIe001-A line has normal karyotype and is free from mycoplasma contamination. PCR analysis confirmed the MUSIe001-A cell line to be a SEA type deletion. MUSIe001-A is a valuable proof of principle model for gene therapy that will facilitate the development of new treatments for affected foetuses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Gene Deletion
Human Embryonic Stem Cells / cytology
Human Embryonic Stem Cells / metabolism*
Humans
Mice
Mice, Nude
alpha-Thalassemia / genetics*