A functionalized dumbbell probe-based cascading exponential amplification DNA machine enables amplified probing of microRNAs

Chem Commun (Camb). 2020 Feb 6;56(11):1681-1684. doi: 10.1039/c9cc08289a.

Abstract

A functionalized dumbbell probe (FDP) based amplification method, termed as a cascading exponential amplification DNA machine (CEA-DNA machine), has been developed to autonomously accumulate single G-quadruplexes (SGQs) and twin-G-quadruplexes (TGQs) for robust fluorescence signal-on probing of miRNA-21.

MeSH terms

  • Benzothiazoles / chemistry
  • Biosensing Techniques / methods
  • Cell Line, Tumor
  • DNA / chemistry*
  • DNA / genetics
  • DNA Probes / chemistry
  • DNA Probes / genetics
  • Fluorescent Dyes / chemistry
  • G-Quadruplexes
  • Humans
  • Inverted Repeat Sequences
  • Limit of Detection
  • MicroRNAs / blood*
  • MicroRNAs / genetics
  • Nucleic Acid Amplification Techniques / methods*
  • Nucleic Acid Hybridization
  • Spectrometry, Fluorescence / methods*

Substances

  • Benzothiazoles
  • DNA Probes
  • Fluorescent Dyes
  • MIRN21 microRNA, human
  • MicroRNAs
  • thioflavin T
  • DNA