Abstract
A functionalized dumbbell probe (FDP) based amplification method, termed as a cascading exponential amplification DNA machine (CEA-DNA machine), has been developed to autonomously accumulate single G-quadruplexes (SGQs) and twin-G-quadruplexes (TGQs) for robust fluorescence signal-on probing of miRNA-21.
MeSH terms
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Benzothiazoles / chemistry
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Biosensing Techniques / methods
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Cell Line, Tumor
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DNA / chemistry*
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DNA / genetics
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DNA Probes / chemistry
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DNA Probes / genetics
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Fluorescent Dyes / chemistry
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G-Quadruplexes
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Humans
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Inverted Repeat Sequences
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Limit of Detection
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MicroRNAs / blood*
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MicroRNAs / genetics
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Nucleic Acid Amplification Techniques / methods*
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Nucleic Acid Hybridization
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Spectrometry, Fluorescence / methods*
Substances
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Benzothiazoles
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DNA Probes
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Fluorescent Dyes
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MIRN21 microRNA, human
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MicroRNAs
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thioflavin T
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DNA